Materials Needed:
Bacillus subtilis
Microbial Bacteria → Through encapsulated capsules
containing Bacillus &Inulin
Concerns:
- Encapsulation with inulin
affecting the culture process
Amazon.com: Vitamatic
Bacillus Subtilis DR 캡슐
당 100억 - 60개 - 소화, 장
및 면역 건강 지원 -
프리바이오틱 이눌린
섬유로 제작 : 건강 및 가정
Pseudomonas fluorescens
→ liquid fertilizers
KCTC 생물자원센터 -
미생물, 동물, 식물 자원
기탁 및 분양
Rhizobium leguminosarum Rhizobium fertilizer 1
2nd fertilizers contain other microbial Rhizobium fertilizer 2
bacterias initiating legume growth,
Amazon.com: Park Seed
therefore need to find a way to
Nature's Aid Garden 토양
observe specific species. 접종제 - 콩, 완두콩 및
수사슴의 토양 비옥도 향상,
3rd → bacteria from Korean collection 더 큰 채소 수확량을 위한
for Type cultures (center) 질소 고정 향상,
45.2m(150피트) 줄의 과립
처리, 246.6g(8.6온스) :
안뜰, 잔디 및 정원
KCTC 생물자원센터 -
미생물, 동물, 식물 자원
기탁 및 분양
Agar plates Bacillus subtilis [NA] 보통한천배지 90mm -
- Nutrient agar (NA) 과학실험키트 | 쿠팡
Pseudomonas fluorescens
- King’s B agar
→encourage fluorescent
pigment production to identify
bacteria
- Nutrient agar
Rhizobium leguminosarum
- Yeast Extract Mannitol Agar
(YEMA) & Congo red
→ specific to identify Rhizobium
General: NA/LB
Plants Vigna radiata (mung bean Pots (number would be
plant/seed) later confirmed)
Soil pH meter Should include Temperature/Soil
Moisture/pH/Meter/Sunlight
Intensity fuctions
Soil Clay soil and sand soil (3kg)
→ sterilised
Acid for pH Sulfuric acid
Calcium hydroxide
Isotonic solution → sterile saline (0.85% NaCl)
Liquid media
Culture process
→ Is this process mandatory?
Disposable spreaders
Sterile pipettes
Sterile inoculating swabs/loops
incubator
Labware/equipments Sterile petri dishes (number would be later confirmed)
UV lamp for Pseudomonas fluorescens (If used)
Disinfectants for cleanup
