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Pneumo Cist Is
Pneumo Cist Is
Geographic Distribution
Worldwide, in humans and animals. Serologic evidence indicates that most healthy children have been exposed by age 3 to 4. Pneumocystis pneumonia (PCP) occurs in immunosuppressed individuals and in premature, malnourished infants.
Life Cycle
These fungi are found in the lungs of mammals where they reside without causing overt infection until the host's immune system becomes debilitated. Then, an oftentimes lethal pneumonia can result. Asexual phase: trophic forms The number 1 replicate by mitosis The number 2 to The number 3. Sexual phase: haploid trophic forms conjugate The number 1 and produce a zygote or sporocyte (early cyst) The number 2. The zygote undergoes meiosis and subsequent mitosis to produce eight haploid nuclei (late phase cyst) The number 3.
Spores exhibit different shapes (such as, spherical and elongated forms). It is postulated that elongation of the spores precedes release from the spore case. It is believed that the release occurs through a rent in the cell wall. After release, the empty spore case usually collapses, but retains some residual cytoplasm The number 4.
A trophic stage, where the organisms probably multiply by binary fission is also recognized to exist. The organism causes disease in immunosuppressed individuals.
Clinical presentation
The symptoms of Pneumocystis pneumonia (PCP) include dyspnea, nonproductive cough, and fever. Chest radiography demonstrates bilateral infiltrates. Extrapulmonary lesions occur in a minority (<3%) of patients, involving most frequently the lymph nodes, spleen, liver, and bone marrow. Typically, in untreated PCP increasing pulmonary involvement leads to death.
The chest radiograph typically shows diffuse infiltrates in both lungs extending from the perihilar region.
Diagnosis
At diagnosis, because the organism does not grow on ordinary media, generally reached by microscopic visualization using appropriate staining sputum, bronchoalveolar lavage or transbronchial biopsy.
Cysts of P. Carinii in lung tissue, stained with methenamine silver and hematoxylin and eosin (H&E). The walls of the cysts are stained black; the intracystic bodies are not visible with this stain.
Gomori methenamine silver stain at high magnification demonstrates cysts of Pneumocystis jiroveci in lung
References:
http://www.cdc.gov/dpdx/pneumocystis/index.html Ruffolo JJ. Pneumocystis carinii Cell Structure. In: Walzer, PD, editor. Pneumocystis carinii Pneumonia. 2nd ed. Marcel Dekker; 1994. p. 25-43.
Cushion MT, Ruffolo JJ, Walzer PD. Analysis of the developmental stages of Pneumocystis carinii in vitro. Lab Invest 1988;58:324-331.