Authors Analit

Type of matrix

Preparasi sampel
Each of meat products (20g) were cut as a slice separately and weighed in beakers.

Analytical technique

Remarks

Gulcemal Sodium Meat (veal) Yildiz , nitrite products Nevin with Oztekin, different Ayca trademarks Orbay, (salami, Filiz sausage,and Senkal bologna)

Deionized water (200mL) was added and blended for 2 min in a laboratory blender.

The suspension was put for 20min in a warm water bath at 50 0 C. After cooling, the specimen was diluted to 250mL and ltered through a Whatman lter paper. the specimen was ltered by using cellulose acetate membrane syringe lter 1mL of the solution was added directly to the 10mL of buffer solution in the electrochemical cell for cyclic voltammetric measurements.

quantitative Standard : nitrite solutions (deoxygenated for 5min with high grade nitrogen) After the background voltammograms in 10mL of phosphate buffer pH 4 had been obtained and recorded. Later, voltammograms were obtained by using different nitrite concentrations. The peak current of the nitrite was obtained at 0.73 V. The accumulation potential of -1.2 V was applied to the working electrode for 5min. Following the accumulation period, after a rest period of 5s, the DPV was recorded from starting potential difference of 0.2V increasing up to 1.2V (with pulse amplitude of 50mV, pulse width of 50 ms, potential step of 4mV, and pulse period of 0.2s). The peak current was measured and recorded as a sample signal.

Authors Cristiana Merusi, Claudio Corradini, Antonella Cavazza , Chiara Borromei, Paola Salvadeo

Analit
Sodium nitrate Sodium nitrite

Type of matrix table sugars and dietary bres

Preparasi sampel By Solid Phase Extraction (SPE) using quaternary amines solid phase on silica support 1.5g of sample +50mL of deionised water Cartridges were conditioned with 2 mL of methanol + 2mL of deionised water,then the 6mL of sample was loaded and eluted with 2mL of NaOH 1M, ow rate 0.5ml/min.

Analytical technique

Remarks

The nal sample solution was diluted 1:1 (v/v) with background electrolyte (BGE) and adjusted at pH 7.0 with phosphoric acid 9M. All samples and standards were ltered through Nylon syringetype lter, 0.2m pore size before analysis.

Instrument : A Model P/ACE MDQ quantitative electropherograph with UV detector was used to perform the analyses Silica capillary :an untreated fused silica capi l l a r y o f 7 5 m and effective length of 21.5 cm Method of injection :by applying a positive pressure on the sample vial of 3.5 kPa at a specied time. Condition of operating voltage : -25kV and temperature was set at 25 0C. Detector : UV Wavelength detection : 214nm Software : 32 Karat TM 5.0 software (Beckman-Coulter) Before use, new capillaries were conditioned with methanol for 2 min, water for 1min, 0.1N hydrochloric acid for 2min, water for 1min,1M sodium hydroxide for 5min , water and the background electrolyte for 20min using a pressure of >150kPa. The capillary was ushed with the running electrolyte for 2min prior to each run.