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Bioreactor Design Overview and Comparisons

This document discusses various types of bioreactors used in cell culture, including chemostats, bubble columns, and airlift reactors. It covers concepts like substrate uptake kinetics in cell culture, observed yields, and mass balances in reactors. Batch, fed-batch, continuous stirred tank (CSTR), and plug flow reactors are compared. Batch reactors can be run as simple batch or with deactivation or cell culture. Fed-batch has advantages like high cell densities and controlled substrate levels but requires operator skill and care to avoid toxin accumulation.

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Vitra Wahyu Pradana
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447 views24 pages

Bioreactor Design Overview and Comparisons

This document discusses various types of bioreactors used in cell culture, including chemostats, bubble columns, and airlift reactors. It covers concepts like substrate uptake kinetics in cell culture, observed yields, and mass balances in reactors. Batch, fed-batch, continuous stirred tank (CSTR), and plug flow reactors are compared. Batch reactors can be run as simple batch or with deactivation or cell culture. Fed-batch has advantages like high cell densities and controlled substrate levels but requires operator skill and care to avoid toxin accumulation.

Uploaded by

Vitra Wahyu Pradana
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPT, PDF, TXT or read online on Scribd

Bioreactor design

Dr. Hadiyanto
Chemostat
Bubble column
Airlift
Product Kinetic in Cell culture
Substrate Uptake
Substrate Uptake with product Formation
Observed Yield
Reactor Comparison
Batch
High operating costs
Batch-to-batch variations
PFR
Difficult to control pH and Temperature
CSTR
Simple pH, Temperature control
Simple catalyst charging and replacement
Concept of Mass Balances
In - out + gen - cons =
accumulation





.
Batch Reactor
Batch
Batch with deactivation
Example
Batch with cell culture
Batch with cell culture
No Maintenance
No Product formation
Example
Fed-Batch
Advantages vs Dis
Advantages:
production of high cell densities due to extension of working time (particularly important in the
production of growth-associated products)
controlled conditions in the provision of substrates during the fermentation, particularly regarding
the concentration of specific substrates as for ex. the carbon source
control over the production of by-products or catabolite repression effects due to limited provision
of substrates solely required for product formation
the mode of operation can overcome and control deviations in the organism's growth pattern1 as
found in batch fermentation
allows the replacement of water loss by evaporation
alternative mode of operation for fermentations leading with toxic substrates (cells can only
metabolize a certain quantity at a time) or low solubility compounds
increase of antibiotic-marked plasmid stability by providing the correspondent antibiotic during the
time span of the fermentation
no additional special piece of equipment is required as compared with the batch fermentation
mode of operation
Disadvantages:
it requires previous analysis of the microorganism, its requirements and the understanding of its
physiology with the productivity
it requires a substantial amount of operator skillfor the set-up, definition and development of the
process
in a cyclic fed-batch culture, care should be taken in the design of the process to ensure that
toxins do not accumulate to inhibitory levels and that nutrients other than those incorporated into
the feed medium become limiting, Also, if many cycles are run, the accumulation of non-producing
or low-producing variants may result.
the quantities of the components to control must be above the detection limits of the available
measuring equipment
Quiz

CSTR
N-series CSTR
Plug Flow Reactor

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