INTRODUCTION : JATROPHA CURCAS L.

OIL
Jatropha curcas L. oil has potential in
production of biodiesel.
Its oil content around 30 - 40%.
Its fatty acid content is around





Fatty Acid Composition Value

Myristic Acid 0.05%
Palmitic Acid 14.07%
Margaric Acid 0.07%
Stearic Acid 6.39%
Arachdic Acid 0.24%
Palmitoleic Acid 0.82%
Oleic Acid 39.90%
Linoleic Acid 34.16%
Arachidonic Acid 0.24%
ENZYME TRANSESTERIFICATION :
BURKHOLDERIA CEPACIA LIPASE
Ethanolysis with immobilized lipase in free
solvent system
Efficient in production of biodiesel of vast
range of vegetable oil
Burkholderia cepacia lipase can be reused
due to the immobilization
Slow reaction
OBJECTIVES
Ethanolysis of Jatropha curcas L. oil by
using enzyme catalyst which is
Burkholderia cepacia

To determine the biodiesel production
through measurement of viscosity
METHODOLOGY
Analysis
Biodiesel a.k.a fatty acid ethyl ester conversion
measured using Brookfield Viscometer LVDV-E

Material
5 batch reactors with Jatropha curcas L. oil, ethanol
and enzyme and repeated one more time

Data
Taken in 4 different parameter which are molar ratio,
enzyme content (wt% of oil), reaction time and water
content (wt% of oil)
PARAMETERS
Molar ratio Enzyme (wt% of oil) Reaction time (hr) Water content(wt% of oil)
Temperature 40 C
pH - 7
RESULT : OBSERVATION
Biodiese
l
Glycero
l
TRANSESTERIFICATION MECHANISM
Ethanol was deprotonated by the enzyme catalyst
to become nucleophile.
The breakage of triglyceride accompanied by the
exchange of acyl acceptor, ethanol R group to
form 3 ethyl ester and 1 glycerol





The existence of heat as well as enzyme catalyst
only increase the reaction rate and does not
change form in the end of the process
1
st
parameter

16.66
14.44
13.57
12.1
13.3
0
5
10
15
20
25
30
35
40
45
0 1 2 3 4 5 6 7
V
i
s
c
o
c
i
t
y

(
m
P
a
.
s
)

Molar ratio
Molar ratio Jatropha Oil Viscosity Biodiesel standard viscosity
2
nd
parameter

14.31
14.01
13.26 13.11
10.68
0
5
10
15
20
25
30
35
40
45
0 10 20 30 40 50 60
V
i
s
c
o
c
i
t
y

(
m
P
a
.
s
)

Enzyme (wt% of oil)
Enzyme concentration (wt%of oil) Biodiesel standard viscosity Jatropha Oil Viscosity
3
rd
parameter

14.55
13.2
11.61
17.79
19.54
0
5
10
15
20
25
30
35
40
45
0 10 20 30 40 50 60 70 80 90
V
i
s
c
o
c
i
t
y

(
m
P
a
.
s
)

Reaction time (hour)
Reaction time Jatropha Oil Viscosity Biodiesel standard viscosity
4
th
parameter

15.25
13.2
18.12
19.8
20.01
0
5
10
15
20
25
30
35
40
45
0 5 10 15 20 25 30
V
i
s
c
o
c
i
t
y

(
m
P
a
.
s
)

Water content(wt% of oil)
Water content (wt% of oil) Jatropha Oil Viscosity Biodiesel standard viscosity
Parameter
Viscosity reduction, cP
= (Jatropha oil - Optimum Jatropha
biodiesel)
Molar ratio 28.35
Enzyme
percentage 29.77
Reaction time 28.84
Water content 27.25
Highest viscosity reduction was found for enzyme
percentage parameter followed by reaction time,
molar ratio and water content percentage
COMPARISON
The hazelnut biodiesel is 8.82 cP while soy oil biodiesel 7.92 cP.
Propertie
s
Refined
jatropha oil
Jatropha oil
biodiesel
Commercial oil
biodiesel
Viscosity
(cP)
25C 40C 25C 40C 40C
50.52 40.45 14.04 10.68 1.9 ~ 6

Fuel properties of jatropha oil based biodiesel in comparison with the jatropha
oil and commercial oil biodiesel
CONCLUSION
Immobilization of lipase based on the experiment
results shows that the optimum parameters are
1:6 molar ratio, 60 hours, 50% enzyme
percentage and 10% water content
The best viscosity value obtained was 10.68 cP.
The calcium alginate beads were appear as
appropriate support for the ester conversion.