E-MOTIF

Presented by:Hamna Khan

E-MOTIF
TheEMOTIFdatabaseisacollectionofmorethan
170000highlyspecificandsensitiveproteinsequence
motifsrepresentingconservedbiochemicalproperties
andbiologicalfunctions.
Theseproteinmotifsarederivedfrom7697sequence
alignmentsintheBLOCKS+databaseandall8244
proteinsequencealignmentsinthePRINTSdatabase
EMotifisveryusefulmethodofidentifyingmotifsin
proteins
Amultiplesequencealignment(MSA)ofaparticularset
ofproteinsissubmittedtoeMotif,whichessentially
searchesforconsensussequence(s)andidentifiesthe
conservativemotifs.

E-Motif
Tools

Emotif
Maker

Emotif
Search

Emotif
Scan

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The eMOTIF-MAKER program converts an

ungapped multiple sequence alignment into a
set of Pareto-optimal discrete motifs for use
by the eMOTIF-SEARCH program and the
eMOTIF-SCAN program.
The resulting set of motifs often reveals
sub-families within the cluster of proteins
that share the region of sequence similarity
covered by the given ungapped multiple
sequence alignment

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eMOTIF-SEARCH: The emotifSEARCH program, previously known

as IDENTIFY.
Given a protein sequence, the eMOTIFSEARCH program suggests families of proteins
to which the query sequence may belong.
The eMOTIF-SEARCH program considers a
protein sequence as potential member of a
protein family, if the sequence contains discrete
motif or motifs that have been generated from an
ungapped multiple sequence alignment of known
member sequences from that family.

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Em
scan

Input emotif regular expression, then

Scan will retrieve protein sequences that
match a user input regular sequences in
a particular sequence database.
A regular expression (sometimes
abbreviated to "regex") is a sequence
of characters that define a search
pattern, mainly for use in pattern
matching

Significances
Efficiency

To analyze Large nucleotide and

proteins sequences, futher,the method
should also minimize the number of
iteration

Accuracy

To identify all locations, including

degenerate occurrences and overlapping
occurrences

User
friendliness

It should be simple to use,platform

independent and display results in an
elegant and easily comprehensible
manner.