pH


*C

UV (280nm)



64500
64500.
;
:
:

 4.


(protein disulfide isomerase PDI).
PDI

.

 1. 70 kd
2
- .
.
40 ,
4 .

1aa = 110 MB
1.5

3.5

30 (4 - 40) aa 45

3-10 aa 45

 3.

.


,
.
;

 m1
,
m2.

a) m1<m2
b) m1=m2
c) m1>m2

12. L-
S L R.
L-
R.

 8.


,
.
;




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/.

,
.

,


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, /.
http://philipkingsley.gr/%CE%95%CE%A0%CE%95%CE%9E%CE%95%CE%A1%CE%93%CE%91%CE%A3%CE%99%CE%91-%CE%9C
%CE%91%CE%9B%CE%9B%CE%99%CE%A9%CE%9D.php

https://www.youtube.com/watch?
v=yJ_pAnKY_c4

DNA, RNA

 DNA RNA

 DNA 5-3





2C


DNA
RNA

RNA

DNA

5- 5


()

5- 5

pApCpG pACG 5ACG3


Watson-Crick
1.

2
.
2. -
(). . (
/ )
3. .
3,4, 10
(34 ), 36
(360/10)
4. 20



DNA
UV



1. ()
2. (m ~72C)
3.

(annealing)
1.
2.

 DNA

Stem-loops

 DNA

1.
2.
3.
4.
5.
6.
7.

20 ,
DNA

DNA
ATP, GTP,
UTP, CTP dATP, dGTP, dTTP, d CTP

()

H DNA

http://www.youtube.com/watch?v=AGUuX4PGlCc&feature=related

http://www.youtube.com/watch?v=UFoDSGvBmAY&feature=related
https://www.youtube.com/watch?v=OnuspQG0Jd0

http://www.youtube.com/watch?
v=4PKjF7OumYo&feature=related

http://www.youtube.com/watch?v=4jtmOZaIvS0&feature=related


RNA

54

55

 ,
1.
2.
3.
4.

RNA
m
t ( 75
r ( )
sn
(r)
(m)
56

 RNA ( 53)
1.
2.
3.
4.
5.

DNA
ATP, GTP, UTP, CTP
Mg2+ Mn2+

RNA

RNA
RNA (
)
3 RNA ,
RNA

57


DNA

58

59

60


;
(, promoter)

61

 RNA

GC rich hairpin loop

polyU

???
RNA

62