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    Windi Mose
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    The regulome is the gene regulatory network in a cell that leads to different patterns of gene expression. It includes membrane-spanning sensors that detect environmental signals and cytoplasmic transcription factors that regulate gene transcription. For example, the transcription factor NRF2 regulates over 35 other transcription factors which in turn influence 63 microRNAs to fine-tune the oxidative stress response. Techniques like bacterial one hybrid, yeast one hybrid, and chromatin immunoprecipitation combined with microarray or sequencing can systematically identify transcription factor-DNA interactions to map out regulons and their target genes.

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    Regulasi genetik

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    The regulome is the gene regulatory network in a cell that leads to different patterns of gene expression. It includes membrane-spanning sensors that detect environmental signals and cytoplasmic transcription factors that regulate gene transcription. For example, the transcription factor NRF2 regulates over 35 other transcription factors which in turn influence 63 microRNAs to fine-tune the oxidative stress response. Techniques like bacterial one hybrid, yeast one hybrid, and chromatin immunoprecipitation combined with microarray or sequencing can systematically identify transcription factor-DNA interactions to map out regulons and their target genes.

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    14 views5 pages

    Regulome

    Uploaded by

    Windi Mose
    The regulome is the gene regulatory network in a cell that leads to different patterns of gene expression. It includes membrane-spanning sensors that detect environmental signals and cytoplasmic transcription factors that regulate gene transcription. For example, the transcription factor NRF2 regulates over 35 other transcription factors which in turn influence 63 microRNAs to fine-tune the oxidative stress response. Techniques like bacterial one hybrid, yeast one hybrid, and chromatin immunoprecipitation combined with microarray or sequencing can systematically identify transcription factor-DNA interactions to map out regulons and their target genes.

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    © All Rights Reserved
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    of 5

    REGULOME

    By Amri
    (Pbi/01333)

    What is regulome?

    Gene regulation is a major cellular process underlying the


    central dogma of molecular biology, encoding the sophisticated
    biochemical cascades that drive cellular behavior and
    development (Ruth, 2014)

    Gene regulatory network in the cell that lead


    to different patterns of gene expression.
    Two-component regulatory systems (2CRS)

    1. Membran spanning sensors


    2. Cytoplasmic transcription factor (Kendall, 2012)

    How regulomes work?


    T
    F

    T
    F
    T
    F

    T
    F

    T
    F

    Cis
    regulatory
    network

    DN
    A
    TFBS
    (Transcription Factor Binding
    Site)
    Non-protein coding DNA
    (ncDNA)

    Membran
    spanning
    sensor
    Cytoplasmic
    transcription
    factor

    Regulate gene
    transcription

    The example of regulome


    NRF2 (NF-E2-related factor 2, NFE212) is a master transcription factor
    of Oxidative and Xenobiotic stress response. NFR2 influencing
    carcinogenesis, inflamation and neurodegeneration.

    35 TFs regulated by NFR2 influence 63 miRNAs that down


    regulate NFR2.
    The presented network may facilitate the development of efficient,
    NFR2 based therapeutic agents (Papp, 2012)

    The techniques to systematically identify


    TF DNA interaction

    Bacterial one hybrid


    system
    The yeast one hybrid
    system
    Chromatin
    immunoprecipitation

    Microarray (ChIP chip)

    Parallel sequencing (ChIP


    seq)

    Identify the regulon and


    determined target genes

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