Periodic Acid Schiff

[PAS] Reaction:
Purpose:
Diagnosis of some acute lymphocytic leukemia
subtypes of AML
M 6

Principle: the stain indicates the presence of

muccoproteins , glycoproteins
and high
molecular weight carbohydrates in blood cells.

Periodic Acid Schiff [PAS]

Reaction

Giant multinucleate late

normoblasts (left). Granular PAS
positivity in proerythroblasts and
homogeneous positivity in the

Interpretation:
Normally all blood cells are (+ve) but

Erythroblasts (-ve)
Diffused stain pattern (Granulocytes)
Granular stain (lymphocytes and monocytes)
Plts

deeply stained
nRBCs (-ve) stain

In diseases:
In CML, lymphosarcoma and Hodgkins` disease (+ve)
staining granules will increase.
nRBCs in M6, thalassemia and other types of anemia may
give [+ve] reaction.

Positive PAS stain

acute
megakaryocytic
leukemia AML, M7.

Positive PAS stain in

ALL

PAS positivity in M6.

Not the intense staining
of the large abnormal
erythroblast.