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Comparative bacteriology

of chronic blepharitis
Novia Isna P
Pembimbing : dr. Rahmat Syuhada, Sp.M

Introduction
Chronic blepharitis is a common and often
difficult
problem for both the physician and the patient.
Much
confusion has surrounded its etiology, which
often
renders treatment futile or frustrating at best.
Three
types of chronic blepharitis have been generally
recognised: Staphylococcal, Seborrhoeic, and
a
mixture of both types.

Purpose
To

evaluate the conjunctival flora


in patients with chronic
blepharitis, and then compared
with a control sample.

Materials and methods


Patients

with chronic blepharitis were


given a careful ophthalmic and
dermatological examination.
Symptoms of at least 6 months and
have not given the therapy. Note the
symptoms, as well as control samples.
Samples were taken from all
individuals are obtained 90 patients
with chronic blepharitis and all
patients were normal.

BACTERIOLOGICAL EVALUATION
Topical

anesthetic drops.
Calcium alginate dampened
brain-heart infusion broth
(Baltimore Biological
Laboratories, BBL).
In the culture at the top and
bottom of the conjunctiva,
eyelashes.
Bilateral done both eyes.

RESULT
Table 1
Clinical classification of chronic blepharitis
of patient classification
staphylococcal
seborrhoeic
alone
with associated staphylococcal
superinfection
with meibomian seborrhoea
with spotty meibomitis
Primary meibomianitis (MKC)

Bacteriology
The

most common frequency isolation of


Staph. aureus.
Coagulase negative Staphylococcus spp,
Corynebacterium spp and
Propionibacterium acnes that are often
isolated from all samples.
Isolation of bacteria from the eyelids lower
than in the conjunctiva, but there was no
significant difference.
When done isolation, Staph. aureus is an
organism with the highest frequency in both
samples.

Aerobic Organisms
In

aerobic organisms were


isolated as :
Streptococcus spp, Micrococcus
spp, Neisseria spp, Branhamella
sp, Corynebacterium spp, Bacillus
spp, and Gram negative
fermentative and nonfermentative.
No significant differences
between the 2 groups

Anerobic Organism
In

anerobic P.acne often found in


the two groups besides
Peptococcus spp and
Peptostreptococcus spp,
Veillonella sp, Fusobacterium spp,
Bacteroides spp, Eubacterium
spp, Lactobacillus spp, and
Clostridium spp.
No significant difference

Therapy
Staph.

aureus have a high


resistance to sulfonamides but
low frequency of resistance to
tetracycline, erythromycin, and
bacitracin. Sensitivity as the drug
neomycin, gentamicin, and
chloramphenicol.

THANKYOU

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