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UNIT 12 Prok - Transcription
UNIT 12 Prok - Transcription
TRANSCRIPTION
Introduction
Transcription Translation
DNA RNA PROTEIN
Replication
direction
Preformed primer is not required
RNA POLYEMERASE:
Complex enzyme made up of multiple polypeptide chain
6 subunits :
2 subunit binding of enzyme to promoter region
nucleotide addition
Rho() dependent
Rho independent
Rho dependent termination
Rho - the termination factor protein
It moves along RNA transcript (>60 nt) , finds the "bubble", unwinds it and
releases RNA chain
mRNA moves away from DNA template as a A-U bp. it detaches and so RNA
polymerase cannot move forward by formation of such structre transcription
ends
Rho independent termination
Rho dependent termination Rho independent termination