BIO-CHROMATOGRAPHY

Types of Chromatography

Adsorption Chromatography
Partition Chromatography
Ion Exchange Chromatography
Molecular Exclusion Chromatography
Affinity Chromatography
Biomolecule. A biomolecule or biological molecule is

molecule that is present in living organisms, including large

macromolecules such as proteins, carbohydrates, lipids,

and nucleic acids,

 Ion Exchange Chromatography
Ion exchange chromatography -- is a separation based on
charge
Used for almost any kind of charged molecules --- large
proteins, small nucleotides and amino acids
Ion-exchange chromatography preserves analyte
molecules on the column based on ionic interactions
Mobile phage buffer, pH and salt concentration---
opposite charged solute ions attracted to the stationary
phage by electrostatic force
Stationary phage resin is used to covalently attach anions
or cations onto it
 Ion-exchange chromatography
The solution to be injected is usually called a sample,
and the individually separated components are
called analytes

It can be used for almost any kind of charged

molecule including large proteins, small nucleotides
and amino acids.

It is often used in protein purification, water

analysis.

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 Principle
Ion exchange chromatography retains analyte
molecules based on ionic interactions.

The stationary phase surface displays ionic

functional groups (R-X) that interact with analyte
ions of opposite charge.

This type of chromatography is further subdivided

into:
1. cation exchange chromatography
2. anion exchange chromatography.

Dr Gihan Gawish 6
 Ion Exchangers

Dr Gihan Gawish 7