Teknik Isolasi

Integral membrane proteins can

be solubilized by non-ionic
detergents
Centrifugation can separate molecules
that differ in mass or density
 Electrophoresis separates
molecules according to their
charge:mass ratio

SDS-polyacrylamide
gel electrophoresis
Two-dimensional electrophoresis separates
molecules according to their charge and their
mass
Separation of molecules by size:
gel filtration chromatography
Separation of molecules by
charge: ion exchange
chromatography
Separation of molecules by specific
binding to another molecule:
affinity chromatography