KjelROC Digestor

Protein
Metode referensi untuk menentukan jumlah protein=nitrogen

Fish
Meat Food
Pharmaceuticals
Grain
Milk

Feed
Water and Waste
Fertilizers
Protein- Different Methods
Kjeldahl Method

+ Accurate
+ Low investment
+ Flexible

-Involves Alkali Chemicals Spectroscopy

- Slow
+ Fast

Dumas/Combustion Method -Depends on Reference Methods

- High Investment
+ Accurate
+ No Alkali

-High Investment
- Expensive Consumables/running costs
- Not as Flexible as Kjeldahl
-Slow when considering Sample Preparation
-Calibrated against Kjeldahl!
Digestion
Digestion
Reference Kjeldahl Distillation Titration
Method
Distillation & Titration
Protein

Dumas/
Combustion
Kjeldahl Method
1883
Johan Kjeldahl
1849 - 1900
Analisa Protein dengan metode Kjeldahl pada dasarnya dibagi menjadi
3 tahap:
1. Destruksi
2. Destilasi
3. Titrasi
Prinsip Kerja Digestion
Tahap digesi merupakan tahap dekomposisi nitrogen dalam sampel
menggunakan asam pekat. Tahap ini disempurnakan dengan
mendidihkan sampel pada asam sulfat pekat. Hasil akhir digesi
merupakan larutan amonium sulfat.
Proses Destruksi

Tambahkan Destruksi
Timbang Masukkan
Asam Sulfat sampai warna
sample diatas kedalam labu
sebanyak 10 ml cairan hijau
kertas sigaret KjelROC
dan katalis 0.5 g jernih
Digestion - Consider
Level of automation
1. Only temp and time, on and off
2. Automatic step program and/or alarms
Good for water analyses and organic samples
Good for notifying when personell is doing other things
3. Automatic switch-off or not
Safety when leaving lab room
4. Automatic cooling of rack
No need to monitor the digestion step

Environmental Concerns
1. Fumes directly in the air or in Fume hood
2. Fumes via exhaust but thereafter directly in the air
Not recommended
3. Fumes via exhaust and thereafter Water Ejector
Cheap solution
4. Fumes via exhaust and Scrubber
Models
Digestor Auto
Everything needed except the automatic lifting of rack (automatic cooling)
Includes one Rack
Digestor 10 with 10 positions or Digestor 20 with 20 positions

Digestor Advanced
Everything needed to run digestion without surveillance
Includes both rack and exhaust
Digestor 10 with 10 positions or Digestor 20 with 20 positions

Scubber
Recommended for environmental concerns (longer lifetime, less vapours in the lab)
Overview

DI-210-A DI-320-A
DI-220-A DI-310-A

DI-110-A DI-010-A DI-020-A DI-040-A DI-050-A

DI-030-A
All LiquidLINE Digestors
Switch-off / alarm signals
Pre programmed &
Customer programmable

Carefully selected materials,

high quality glass Tube Racks for silent operation

Standard size Test Tubes compatible

Reliable and Safe
with several other brands
Digestors Benefits
All models with
Pre programmed & Customer
programmable
Standard size Test Tubes
compatible with several other
brands
Tube Racks for silent
operation
Switch-off / alarm signals
Carefully selected materials,
high quality glass
Exhaust manifold
Safe
Comparing - USP
Unique Selling Points
Be n e fit
Security Systems - hot plate warning,
software surveillance of functions
Silent Technology on racks
Ad va n ta g e Co mp e tito rs
Safe working environment Some local manufactures saves
cost by removing these features
Good working environment with very little noise when -
moving racks with tubes
Comparing

Additional Benefits
Be n e fit Ad va n ta g e C o mp e tito rs
Automatic Programs Easy and fast to initiate a digestion Not all Semi-Auto Digestors have this

Only high quality materials used
High quality exhaust racks
Fully automatic Motor rack in Digestor
Advanced
Manual Programming of temperature, time
and end-behaviour
Saves cost in maintenance Not all Digestors resulting in broken
glass and/or rust
Lowers cost for maintenance - robust design of seals Not all Digestors
reduces amount of exchanges, saves cost
Saves time when no surveillance of process is necessary -
and there is automatic cooling of samples
Easy to customize your Digestor Not all Digestors
Alarm signal simplifies surveillance of process - saves time
Competition
Competitor Models
Bchi SpeedDigester K-425/436 Basic IR Digestor - no motorrack
Bchi SpeedDigester K-439 Basic IR Digestor - no motorrack
Bchi Digest Automat K-438 Motorrack - corresponds to KjelROC Digestor Advanced
No Motorrack - DK 6-8-20 (250 ml tubes) competes to KjelROC Digestor Auto
VELP DK-Series
(6-6/48-8-20-20/26-42/26 pos.)

DKL 8-12-20 competes to KjelROC Digestor Advanced

VELP DKL-Series
(8-12-20-42/26 positions)

Basic IR Digestor - no motorrack

Gerhardt TurboTherm
(4-6-12 positions)

Gerhardt KjeldaTherm Basic
(4-6-12 positions)
Gerhardt KjeldaTherm Automatic
(4-6-12 positions)
No Motorrack
Time-control of program optional (not included as standard)
competes to KjelROC Digestor Auto
Includes Motorrack
competes to KjelROC Digestor Advanced

FOSS Digestor Basic Not possible to program, very basic model

(8, 20 positions)
FOSS Digestor Auto Rack competes to KjelROC Digestor Auto
(8, 20 positions)
FOSS Digestor Auto Lift competes to KjelROC Digestor Advanced
(8, 20 positions)