T VN

Trong cng nghip sn xut ng, bn cnh ng

l sn phm chnh, chim mt lng khng nh l r
ng-sn phm ph
R ng l loi nguyn liu r tin, d kim, l mi
trng giu ngun carbon, cung cp ngun dinh
dng cho vi sinh vt pht trin s dng nhiu trong
cng ngh ln men to ra nhiu sn phm
Ln men lactic c ng dng rng ri trong cng
ngh thc phm, lactic acid trn nhiu lnh vc nh
bo qun v ch bin thc phm, trong y hc, mi
trng, m phm.v.v.
nhu cu ngy cng nhiu v ngun lactic acid
Lactic acid v qu trnh ln men lactic: ng dng rng ri
trong cng nghip thc phm v nhiu lnh vc khc
nh y t, m phm, thuc da. Nhu cu v lactic acid
ngy cng cao iu thc y ngi ta quan tm
nghin cu v tm cch to ra lactic acid nhiu m gi
thnh r.
R ng: sn phm ph - chim mt lng khng nh
ca ngnh cng nghip sn xut ng, (1/3 sn lng
ng sn xut). Nu khng tn dng ngun r ng
ny s l mt lng ph rt ln, ng thi lng r ng
nu thi ra s gy nhim mi trng. Chnh v vy, r
ng cng c tn dng sn xut nhiu sn
phm khc.
 R ng:loi nguyn liu r tin, d kim,
l mi trng giu ngun carbon, cung
cp ngun dinh dng cho vi sinh vt
pht trin nn c s dng nhiu trong
cng ngh ln men to ra nhiu sn
phm. C th ni y l mi trng thch
hp s dng ln men thu lactic acid
mt cch hiu qu m g thnh r hn
 MC CH TI

Thit lp mt s thng s cng

ngh trong ln men sn xut lactic
acid quy m phng th nghim

To tin trin khai sn xut

lactic quy m ln hn
NI DUNG NGHIN CU
nh hng
ca nhit
nui cy

Kh nng sinh
acid lactic bi
chng P.
pentosaceus T13

Kho st s thay i hm lng ng kh

trong qu trnh ln men
I TNG V PHNG
PHP NGHIN CU
 I TNG

R ng

Chng vi khun Pediococcus

pentosaceus T13
Phng php nghin cu
Phng php vi sinh
Phng php ha sinh
Phng php vt l
Phng php ton hc
Mt s phng php b tr th
nghim lin quan
Phng php nghin cu
Dch t bo vi khun
ging 1ml mi
Phng php trng (0,1% Pepton,
hot ha ging 0,5% NaCl, pH= 6,2)
trong 1 gi.

Phng Phng php

Dch ging mi
trng MRS lng, pH=
php vi nui cy, tng
sinh
6,2
37oC trong 24 gi
sinh
Dch tng sinh c
cy chuyn mi
Phng php trng MRS lng,
gi ging 37oC , trong 24 gi
Gi lnh 4oC
Phng php nghin cu
Phng php
ha sinh

Xc nh hm lng lactic
Xc nh hm lng ng
acid bng phng php sc
tng ca r ng
k lng cao p

Nguyn tc C s l thuyt
chung

Tin hnh Tin hnh

Phng php nghin cu

Phng php xc
nh sinh khi t bo
Nguyn tc:
bng cch o mt
quang (OD)
Phng
php vt l
Xc nh pH ca
mi trng nui cy
bng my o pH
in t
Phng php nghin cu
Phng php ton hc
S liu c tnh trung bnh, lch chun v x l
ANOVA bng phn mm SPSS16.0
 Qu trnh b tr th nghim
R ng

X l r ng

Kho st nh hng ca mt t bo gieo cy ban u n

kh nng sinh lactic acid (105CFU/ml, 106CFU/ml, 107CFU/ml
)

Kho st nh hng ca nhit nui cy n kh nng sinh

lactic acid
(t = 37oC, 40oC, 43oC)

Kho st nh hng ca hm lng mi trng r ng n kh

nng sinh lactic acid (hm lng r ng 6%, 8%, 10%, 12%, 14%)

Kho st nh hng ca thi gian ln men khc nhau n

kh nng sinh lactic acid (T= 12 gi, 24 gi, 36 gi, 48 gi)
 KT QU NGHIN CU
Kt qu phn tch hm lng ng tng trong r ng
Kt qu nghin cu nh hng ca mt t bo nui cy
n kh nng sinh lactic acid ca P. pentosaceus T13
Kt qu nghin cu nh hng ca nhit nui cy n
kh nng sinh tng hp lactic acid ca chng P.
pentosaceus T13
Kt qu nghin cu nh hng ca nng r ng n
kh nng sinh lactic acid ca chng P. pentosaceus T13
Kt qu nghin cu nh hng ca thi gian nui cy n kh
nng sinh tng hp lactic acid ca chng P. pentosaceus T13
S thay i hm lng ng trong qu trnh ln men
Kt qu phn tch hm lng lactic acid bng phng php
sc k lng cao p
Kt qu phn tch hm lng ng tng trong r ng

R ng (1ml)

Ha long bng nc
ct(100ml) Thc hin phn ng mu
bng DNS
Thy phn bng HCl
70oC, 10 pht si 10 pht

Trung ha bng NaOH 10 pht

nh mc 100ml B sung 2ml nc ct

Lc dch trung ha So mu bc sng

540nm
1. Kt qu phn tch hm lng ng tng trong r ng

th ng
chun glucose
OD o Hm lng r
c ng

Hm lng
Mu
Gi tr ng (mg/ml)
Mu
OD 1 245,79
1 0,190
2 253,75
2 0,197

3 0,198 3 243,86

Trung
bnh 247,8
2. Kt qu nghin cu nh hng ca nhit nui cy n
kh nng sinh tng hp lactic acid ca chng P.
pentosaceus T13

0 gi 12 gi 24 gi 36 gi 48 gi

pH
6.5 6,2a 6,2a 6,2a

5.5

5 4,58b 4,5b
4,49b

4.5
3,76c
4 3,61c 3,65c
3,29d 3,2d
3.5 3,18e 3,12d 3,15d
3,07d

2.5
37 40 43

Thi gian ln men (oC)

3. Kt qu nghin cu nh hng ca mt t bo nui cy n
kh nng sinh lactic acid ca Pediococcus Pentosaceus T13

pH 0h 12h 24h 36h 48h

6.5
6,2a 6,2a 6,2a 6,2a 6,2a

5.5

5
4,69b
4,56b 4,49b
4,43b 4,45b
4.5

3,88c
4 3,8c
3,61c 3,67c 3,66c
3,4d
3,34d 3,3d
3.5 3,24e 3,25d d
3,18e 3,12d 3,19 3,18e
3,07d
3

2.5
6% 8% 10% 12% 14%
Hm lng r ng
 4. Kt qu nghin cu nh hng ca mt t bo nui
cy n kh nng sinh lactic acid ca P. pentosaceus T13

Mt t bo
gieo cy ban
u
105 106 107
CFU/ml CFU/ml CFU/ml
Thi gian

0 gi
6,2a 6,2a 6,2a

12 gi 5,12b 4,5b 4,48b

24 gi 4,2c 3,62c 3,57c

36 gi 3,54d 3,12d 3,10d

3,28e 3,06d
48 gi 3,07d
Kt qu nghin cu nh hng ca thi gian nui
cy n kh nng sinh tng hp lactic acid ca
chng P. pentosaceus T13

pH
6.5 6,2a pH ti 10%

5.5

5 4,49b
4.5

4 3,61c

3.5 3,12d 3,07d

2.5
0 gi 12 g 24 gi 36 gi 48 gi

Thi gian ln men

S thay i hm lng ng trong qu trnh ln
men

30

24,78a
Hm lng ng (mg/ml)
25

20
16,35b
15

9,72c
10

5,18d
5
4,62e

0
0 gi 12 gi 24 gi 36 gi 48 gi

Thi gian ln men

Kt qu phn tch hm lng lactic acid
bng phng php sc k lng cao p
Kt qu phn tch hm lng lactic acid
bng phng php sc k lng cao p
Kt qu o hm lng lactic acid mu dch r ng c
hm lng 10%, sau thi gian 36 gi ln men nhit
40oC bng phng php sc k lng cao p cho ta kt
qu 14,5 g/l.
KT LUN V KIN NGH

Kt lun

Kin ngh
 Kt qu
- Ta chn mt t bo 106(CFU/ml) tin hnh qu trnh ln
men s cho ta c cc kt qu ti u nh mong nun
- Hm lng r ng thch hp i vi s pht trin v sinh
tng hp lactic acid vi hm lng cao ca chng Pediococcus
Pentosaceus T13 l10%.
- Xc nh c thi im thch hp thu lactic acid ca
chng Pediococcus Pentosaceus T13 trong mi trng r ng
c nng 10% l sau 36 gi.
- Xc nh c nhit nui cy ti thch hp chng
Pediococcus Pentosaceus T13 sinh tng hp lactic acid cao l
40oC
-Kt qu phn tch hm lng ng tng trong r ng l
247,8 g/l
-Trong qu trnh ln men hm lng ng gim theo thi gian.
V mc gim hm lng ng trong mi trng ln men
tng thch vi s thay i pH theo thi gian cc nng r
ng khc nhau
5.2. Kin ngh
-Tip tc nghin cu ti u ho cc iu kin
khc chng Pediococcus Pentosaceus T13 c kh
nng sinh tng hp lactic acid cao nh ngun
nit.
Kho st kh nng sinh tng hp lactic acid khi
kt hp mt vi chng vi khun lactic khc nhau
thc hin qu trnh ln men mi trng r
ng.
Kho st qu trnh ln men cc hm lng r
ng cao hn v mi trng c b sung mt
s thnh phn khc vo r ng.
M rng nghin cu sn xut lactic acid t r
ng quy m ln hn