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  • Microbiology Laboratoric Test

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    indri
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    The document describes the procedure for Ziehl-Neelsen staining used to identify acid-fast bacteria under a microscope. It involves collecting a tissue sample from the ear by cleaning the skin with alcohol, pressing on the earlobe until pale, and making a cut with a scalpel. The sample is then stained using Ziehl's carbolfuchsin dye, methylene blue, and acids to identify red-stained acid-fast bacteria under a microscope.

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    Microbiology laboratoric test

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    Ziehl Neelsen Staining

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    The document describes the procedure for Ziehl-Neelsen staining used to identify acid-fast bacteria under a microscope. It involves collecting a tissue sample from the ear by cleaning the skin with alcohol, pressing on the earlobe until pale, and making a cut with a scalpel. The sample is then stained using Ziehl's carbolfuchsin dye, methylene blue, and acids to identify red-stained acid-fast bacteria under a microscope.

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    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
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    11 views17 pages

    Microbiology Laboratoric Test

    Uploaded by

    indri
    The document describes the procedure for Ziehl-Neelsen staining used to identify acid-fast bacteria under a microscope. It involves collecting a tissue sample from the ear by cleaning the skin with alcohol, pressing on the earlobe until pale, and making a cut with a scalpel. The sample is then stained using Ziehl's carbolfuchsin dye, methylene blue, and acids to identify red-stained acid-fast bacteria under a microscope.

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    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
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    of 17

    Microbiology

    Laboratoric Test
    Specimen collection for Ziehl-Neelsen
    Staining

    Desinfeksi kulit dengan alkohol 70 %


    Specimen collection for Ziehl-Neelsen
    Staining

    Tekan cuping telinga dengan menggunakan dua jari sampai pucat


    Specimen collection for Ziehl-Neelsen
    Staining

    Sayat dengan menggunakan blade scalpel No. 15


    Specimen collection for Ziehl-Neelsen
    Staining

    Putar blade scalpel 90o


    Specimen collection for Ziehl-Neelsen
    Staining

    Kumpulkan bubur jaringan, jika ada darah prosedur diulangi lagi


    Ziehl-Neelsen Staining
     Material
     Ziehl’s carbolfuchsin  microscope
    prepared with either
     bibulous paper
    Tergitol No. 4 (a drop per
    30 ml of carbolfuchsin) or  paper toweling
    Triton-X (2 drops per 100  lens paper and lens
    ml of carbolfuchsin). cleaner
     alkaline methylene blue  immersion oil
     acid-alcohol  staining racks
     clean glass slides  1-ml pipettes with pipettor
     inoculating loop  Hand gloove
     hot plate
    Ziehl-Neelsen Staining
    Ziehl-Neelsen Staining
    Ziehl-Neelsen Staining
    Ziehl-Neelsen Staining
    Ziehl-Neelsen Staining
    Ziehl-Neelsen Staining
    Ziehl-Neelsen Staining
    Ziehl-Neelsen Staining

    Examination the preparation under


    microscope ( x100) for confirmation
    Microscopic result for Ziehl-Neelsen
    Staining

    Acid Fast Bacteria

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