DIAGNOSTIC STUDY CIRCLE

"Myiasis And Observation

Of Maggots by
Posterior Spiracles"

Submitted By,
Gagana L.
HVK 1517
 CONTENTS
TOPIC

INTRODUCTION

CLASSIFICATION OF MYIASIS

CLINICAL SIGNS OF INFECTED ANIMAL

MATERIAL AND METHOD

IDENTIFICATION AND SPECIATION

OBSERVATION

CONCLUSION AND RESULT

TREATEMENT

REFFERANCE
 ABSTRACT
• MYIASIS is being a common clinical condition
leads to serious morbidity somewhile.
• The speciation is done based on posterior
spiracles of maggots to know which fly is
responsible.
• According to examined sample lucilia is major
than chrysomia and musca.
 MYIASIS INTRODUCTION
• MYIA Greek word for ‘fly’
• Hope in 1940 coined it and it refers to animal
disease due to fly larvae.
• Zumpt in 1965 defined myiasis as ‘infestation
of live animal with the larvae of dipteran fly
which atleast for a certain period feed on the
host dead or living tissues, liquid body
substance or ingested food
In sheep it is reffered to as strike or blow fly
myiasis. Generally caused by larvae of
CHRYSOMIA, LUCILIA, PHORMIA AND
CALLIPHORA.
 Classification of MYIASIS
• ANATOMICAL • PARASITOLOGICAL
• Sanguinivorous • Obligatory myiasis
• Dermal/ subdermal • Facultative myiasis
• Nasopharyngeal • Accidental or psuedo
• Intestinal myiasis
• urogenital • Miscellaneous myiasis
CLASSIFICATION OF FLIES

Primary flies
Secondary flies
Tertiary flies
 lucilia chrysomia musca

Oestrous ovis gastrophilus stomaxys

 Clinical signs of infected animal
• Irritation animal stands with head down
• Does not feed, moves away from flock
• Attempts to bite affected area
• If lession is in tail region animal stamps or jerk its
hind legs wags its tail
• Trimming of wool reveals presence of maggots.
• Leads to malnutrition, loss of production , death.
• Animal does not feed properly
• Weak and debilitating.
• It causes toxiemia and septiceamia
• Finally death.
 Materials and methods
• Live myiasis shrink if placed directly in
ethanol.
• A mixture of three parts of ethanol of 90%
ethanol plus of glacial acetic acid as a
preservatives will not cause shrinkage .
• This is useful for field work but in the
laborotary it is a simplest to kill the larvae in
water at about 80 c and then preserve them
in the usual 75% ethanol.
 Procedure For Sample Collection
• Leave the live specimen in 12-24 hrs or untill
the specimen become lighter on color.
• After rinsing in water, replace with acidulated
70% alcohol, dehydrate, clear and mount in
usual manner.
• The period of KOH can shortened considerably
by heating solution containing the specimen.
• This process should be watched since the
chitin is destroyed by overheating.
Collect maggots in
10% KOH
Clear transparent
Posterior spiracles
• This method is that it reduces the number of
time the specimen are handled, which
minimizes the chance of damaging them.
• Cut the posterior part of larvae.
• Mount with normal DPX medium
• Observe under 4X.
 Identification of larvae
There are diff spp...

• Musca spp
• Stomaxys spp
• Lucilia spp
• Chrysomia spp
• Gastrophilus spp
• Oestrus ovis
 Musca spp
• Dark brown ‘D’ shaped spiracles.
• Well developed peritreme with median
button.
• Each spiracles has 3’m’ shaped torturous
winding slits.
 Stomaxys spp
• Triangular spiracles
• Button is present at the center
• Each spiracles has 3 ‘S’ shaped winding slits
 Lucilia spp
• Peritreme is narrow and button present
• Presence of 3 longitudinal slits
• Peritreme show a single inner projection
between two slits.
 Chrysomia spp
• Peritreme incomplete
• Button absent
• presence of 3 longitudinal slits
 Gastrophilus spp
• Bean shaped spiracles
• Presence of 3 wavy slits
 Oestrus ovis
• Large D shaped chitinous dark spiracles
• A central button with numerous radiating
slits.
 Conclusion
• Lucillia = 66.66%
• Chrysomia = 16.66
• Musca = 16.66
musca lucilia

chrysomia
 Examined samples
species Region Flies
cattle Loin region Lucilia
Cattle Vulva Musca
Dog Ear Chrysomia
Dog Jaw Lucilia
Dog Loin Lucilia
Cattle back Lucilia
 Treatement
• Turpentine oil is used to wound infested with
maggots
• Ivermectin on wound is also used
• Maggots being common case this has to be
controlled by management of wound areas
• Or else by using antireppelents to avoid fly
laying eggs
• This can be controlled by using neem oil in
farm level or farmers level.
 Referance

• Veterinary clinical parasitology

By Anne M zajak
Gary A Conby

VPA211 Practical manual