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Assignment 7.13
Assignment 7.13
IM CC BUGNA
PGI EVANGELISTA
Lowenstein Jensen Medium
(LJ Medium)
• LJ Medium Base :
L-Asparagine = 3.6 g
Monopotassium Phosphate = 2.5 g
Magnesium Sulfate = 0.24 g
Sodium Citrate = 0.6 g
Malachite Green = 0.4 g
Potato Flour = 30 g
• Supplements:
Glycerol = 12 ml
Egg suspension = 1000 ml
Latent tuberculosis infection (LTBI)
• Several weeks after infection, two host responses to M.
tuberculosis develop: a macrophage-activating CMI response
and a tissue-damaging response.
• The macrophage-activating response is a T cell–mediated
phenomenon resulting in the activation of macrophages that
are capable of killing and digesting tubercle bacilli.
• The tissue-damaging response is the result of a delayed-type
hypersensitivity (DTH) reaction to various bacillary antigens; it
destroys unactivated macrophages that contain multiplying
bacilli but also causes caseous necrosis of the involved tissues.
• Although both of these responses can inhibit mycobacterial
growth, it is the balance between the two that determines the
forms of TB that will develop subsequently.
Source: Harrison’s Principle of Medicine 19th Edition
• With the development of specific immunity
and the accumulation of large numbers of
activated macrophages at the site of the
primary lesion, granulomatous lesions
(tubercles) are formed.
• Initially, the tissue-damaging response can
limit mycobacterial growth within
macrophages.