Molecular Biology

(BT3012)
 No. of credits: 3
Slot: G

Class Time Table:

Monday 1:00 PM
Thursday 10 AM
Friday 9 AM
Extra class: Wednesday 4:50 PM
 Course content
Basic concepts, strategies and applications of the art of Molecular Biology

Part I: The basis of molecular biology

(the flow of genetic information; structure, isolation and quantification of
nucleic acids; gel electrophoresis; nucleic acids hybridization; DNA
sequencing; restriction enzymes; overview of recombinant DNA technology;
chemical syntheses of nucleic acids)

Part II: The methodologies of gene manipulation

(host cells and vectors; cloning strategies; Polymerase Chain Reaction; site-
directed mutagenesis; selection and analysis of recombinants)

Part III: Applications of molecular biology

(making and altering proteins in different expression systems; medical and
forensic applications; transgenic plants and animals; animal cloning; ethical
aspects)
 Grading criteria:

I Quiz: 30 marks

Assignments/Class Tests: 20 marks

End Sem: 50 marks

 References:

1. Biochemistry – Lubert Stryer

2. Genes – Benjamin Lewin
3. From Genes to Clones – E.-L. Winnacker
4. Molecular Cell Biology- Harvey Lodish