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Plasmid Ti

Materials
• pGEMTeasy-MmPMA: ampicillin resistence gene
• pENTR/D-TOPO: kanamysin resistence gene
• pGWB502 : spectinomysin and hygromysin
resistence gene
• E. coli DH5α
• E. coli DH1: resistence to kanamysin
• A. tumefaciens LBA4404: resistence to streptomysin
• N. tabacum SR1
Contoh tanaman jalaipam yang beregenerasi
3 minggu di media seleksi hyg 40 mg/L

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