Gamma Radiosensitivity Study in Rice

(Oryza sativa)
Nurul Nadia binti Jaafar, Faiz bin Ahmad
Malaysia Nuclear Agency, and Address.

COMPANY BACKGROUND
Malaysia Nuclear Agency is a government institution that has a role to introduce and promote application of nuclear science and technology for national development. Malaysia Nuclear Agency was established
in 19 September 1972 and it was known as Centre of Application Nuclear Malaysia (CRANE). Later on the name was changed to Malaysia Institute for Nuclear Technology Research (MINT) in August 1994. On 28
September, MINT was given a new identity – Malaysia Nuclear Agency (Nuclear Malaysia). Malaysia Nuclear Agency (Nuclear Malaysia) is a reputable agency in research and development (R&D) in science and
technology. From the time when it was established, Nuclear Malaysia has been given the responsibility to introduce and promote nuclear science and technology for national development and until present.
Nuclear Malaysia also plays a pivotal role in providing quality and best-in-class research towards comprehensively generating new technologies to meet the needs of nuclear technology’s variety of application.

INTRODUCTION
Rice is the principle staple crop of Asia and any deterioration of rice production systems through climate change would seriously impair food security in this continent. The existence genetic varieties with useful
traits are needed for plant breeding in improving crops. However, the traits that always been desired always lacking. In Malaysia, lack in rice variety that resists to bacterial leaf blight (BLB) disease is one the
major problem that constraint rice production. MR 284 rice variety is newly released in 2015 by MARDI. It is highly yield variety, however it’s resistant towards bacterial leaf blight is moderate. Therefore,
mutation breeding will acts as an alternative to overcome this problem. Mutation breeding is a technique that is used to create new varieties by generating and utilizing genetic variability through chemical and
physical mutagenesis.

1. To determine optimum gamma irradiation dose (LD50 and LD20) for MR 284 variety
OBJECTIVE
2. To determine the effect of gamma rays on morphological characteristics of paddy seedling derived from irradiated seeds.

METHODOLOGY RESULTS AND DISCUSSION

Acute Gamma Irradiation

30 paddy seeds for each Petri dishes contained paddy seeds Soaked in fungicide for 24 hours
treatment placed in petri dishes placed in Biobeam to be irradiated.

LD50 and LD20 for Shoot Length

.7 3
109
S hoot length (cm )

49
91.

24
73.

00
Placed in germination Conducted sandwich blotter 55.

chamber technique 36.

76

51
Shoots and roots length 18.

measured on 7th and 14th day 0 .2

7
0.0 183.3 366.7 550.0 733.3 916.7 1100.0

Dose (Gy)
Chronic Gamma Irradiation

Seeds germinated in
petri dish for 1 week

Soil prepared in pots Germinated in tray for 1 week

Seedlings planted & placed in paddy house for

control treatment & in Gamma Green House at
Plant height measured at 7th, different rings
14th and 21st day

CONCLUSION
• The LD50 for shoot length of acute irradiation is 390.74 Gy and the LD20 is 257.46 Gy.
• The gamma irradiation (acute & chronic) can caused reduction in all morphological traits such as shoot length, root length and plant height.

REFERENCES ACKNOWLEDGEMENT
• Director of Agrotechnology and Biosciences Division (Dr. Abdul Rahim Harun)
• En Faiz bin Ahmad (Supervisor in Malaysia Nuclear Agency)
• Staffs of Plant Improvement Group
• Dr. Nor’aishah Hasan (Supervisor in UiTM)
• UiTM trainees in Malaysia Nuclear Agency