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Blotting Techniques: The Muscular System
Blotting Techniques: The Muscular System
Blotting Techniques: The Muscular System
Martini / Bartholomew
BLOTTING TECHNIQUES
7 The Muscular
System
By
Abdullah Al-Hatami
PowerPoint® Lecture Outlines
PhD
prepared by Alan Magid, Duke student
University
College of Medicine
University of Kufa
What is blotting?
Blotting technique
1. A protein sample is
subjected to
electrophoresis on an
SDS-polyacrylamide
gel.
2. Electroblotting
transfers the
separated proteins
from the gel to the
Cont…
3. The blot is incubated with a
generic protein (such as milk
proteins or BSA) which binds to any
remaining sticky places on the
nitrocellulose.