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Phylogenetics
RNA functions
• Genomes
• many viruses have RNA genomes
single-stranded (ssRNA)
e.g., retroviruses (HIV)
double-stranded (dsRNA)
Catalytic
RNA in the ribosome has peptidyltransferase activity
• Enzymatic activity responsible for peptide bond formation
between amino acids in growing peptide chain
• Also, many small RNAs are enzymes
"ribozymes“
Regulatory
L Samaraweera 2005
Outline
RNA Structure
Helices are inferred when two or more base pairs occur adjacent to one another
Single stranded bases within a stem are called a bulge of bulge loop if the single
stranded bases are on only one side of the stem.
If single stranded bases interrupt both sides of a stem, they are called an internal
(interior) loop.
RNA secondary structure representation
..(((.(((......))).((((((....)))).))....)))
AGCUACGGAGCGAUCUCCGAGCUUUCGAGAAAGCCUCUAUUAGC
RNA structure prediction
Two primary methods for ab initio RNA secondary
structure prediction:
S (i 1, j ) ri unpaired
S (i, j 1) rj unpaired
S (i, j ) max
S (i 1, j 1) e( ri , rj ) i, j base pair
max S (i, k ) S (k 1, j ) i, j paired , but not to each other
i k j
To compute this efficiently, we need to make sure that the scores for
the smaller sub-regions have already been calculated
Other methods
A C
D
time
A B C D B
Several methods:
• Distance-based or Clustering methods
• Parsimony
• Likelihood
• Bayesian
Types of phylogenetic analysis methods
Organisms Trees
1 1
2 1
3 3
4 15
5 105
6 945
7 10,395
8 135,135
9 2,027,025
10 34,459,425
15 213,458,046,676,875
30 4.9518E38
Searching for the optimal tree…
50 2.75292E76
Support for phylogenetic methods