DISSOLVED OXYGEN

ANALYSIS

SUSHMA KUMARI
R.D.S. COLLEGE, MUZAFFARPUR
DISSOLVED OXYGEN ANALYSIS

BY

WINKLER METHOD
 APPARATUS

BOD(Biological Oxygen Demand) Bottles

Measuring Cylinder

Pipette

Conical Flask
 Winkler A

CHEMICALS
Winkler B

Concentrated Sulphuric Acid

(H2SO4)

Sodium Thiosulphate

Starch Indicator
Winkler A (Manganese Sulphate)

100g of MnSO4
mixed in 200ml of distilled
water
 Winkler B (Alkali-iodide-azide)

100g of KOH (Potassium

Hydroxide) and 50g of KI
(Potassium Iodide) mixed in
200ml of distilled water
 Sodium Thiosulphate

25g Sodium thiosulphate

mixed in 1litre of
distilled water
 Starch Indicator

1g of starch mixed in
100ml of distilled
water
 PROCEDURE
Carefully fill a 300ml glass Biological Oxygen Demand
(BOD) stoppered bottle brim-full with sample water.
Immediately add 1ml of Winkler A to the collection
bottle by inserting the pipette just below the surface of
the liquid.
Add 1ml of alkali-iodide-azide (Winkler B) reagent in the
same manner.
Stopper the bottle with care to be sure no air is
introduced.
Mix the sample by inverting several times.
Add 1mL of concentrated sulfuric acid via a pipette held
just above the surface of the sample.
Mix the sample by inverting several times.
At this point, the sample is "fixed" and
can be stored for up to 8 hours if kept
in a cool, dark place.
In a Conical flask, titrate 50 mL of the sample
with sodium thiosulfate to a pale straw colour.
Titrate by slowly dropping titrant solution from a
pipette into the flask and continually stirring the
sample water.
Add 3-5 drops of starch solution so a blue colour
forms.
Continue slowly titrating until the sample turns
clear.
As this experiment reaches the endpoint, it will
take only one drop of the titrant to eliminate the
blue colour.
mg/l DO2 =
ml of Titrant × 0.025 × 1000 × 8 /
Volume of Sample