𝜸 − 𝑮𝑻 𝒍𝒊𝒒𝒖𝒊𝒄𝒐𝒍𝒐𝒓

Colorimetric Test
 NAMA ANGGOTA :

Salma Mardhiyah (P27834118009)

Annisa Akstari ‘Ilma F. (P27834118028)
Chori Khotul Ula (P27834118032)
Alissa Qotrunnada (P27834118040)
Methode

Kinetic colorimetric method for the

determination of 𝜸 − 𝑮𝑻 activity according to
Persijn & van der Silk. Standardised againts
recomended IFCC method .
C
O
N
T
E
N
T
S
Reagent Preparation anad Stability
Procedure 1 with reagent start
1. The reagents are ready for use

2. The reagents are stable, even after opening, up to the stated

expiry date when stored at 2-8˚C. Contamination of regaents
mest be avoided.
Procedure 2 with sample start
1. 12033 and 12023 : Pour the entire contents of one bottle substrate into
one bottle buffer, mix troughly.
2. 12213 : Pippet 1 mL from bottle substrate into one bottle buffer , mix
troughly.
3. 12013 : Pippet 2 mL from bottle substrate into one bottle buffer , mix
troughly
The working reagent is stable for 6 weeks at 2-8 ˚𝑪 and 5 days at 15 – 25 ˚𝑪
Procedure 1 with reagent start
1. The reagents are ready for use
2. The reagents are stable, even after opening, up
to the stated expiry date when stored at 2-8˚C.
Contamination of regaents mest be avoided.

Procedure 2 with sample start

1. 12033 and 12023 : Pour the entire contents of one
bottle substrate into one bottle buffer, mix troughly.
2. 12213 : Pippet 1 mL from bottle substrate into one
bottle buffer , mix troughly.
3. 12013 : Pippet 2 mL from bottle substrate into one
bottle buffer , mix troughly
The working reagent is stable for 6 weeks at 2-8 ˚𝑪 and 5
days at 15 – 25 ˚𝑪
SPECIMEN

Specimen : Serum and EDTA plasma

Note:
No Loss of activity within 7 days
at ± 4̊C anad 20-25 ℃
A
Wavelenght : Hg 405 nm (400-420 nm)
S Optical path : 1 cm
S Temperature : 25˚C, 30˚C, or 37˚C
Measurement : againts air
A
Warm the reagent and the cuvettes to the desired
Y temperature. Temperature must be kept constant
( ± 0,5 ̊C ) for the duration of the test
Performace Characteristics

Linearity

If the arbsorbance change per minute exceeds 0,200 at Hg

405 nm dilute 0,1 mL of the sample with 0,5 mL physiological
saline (0,09%) and repeat the assay using this dilution multiply
the result by 6. Typical performance data can be found in the
Verification Report.
Quality Control

1. All control sera with γ-GT values determined by ths method

can be employed.

2. We Recomended to use our animal serum based HUMATROL

or our human serum based SERODOS quality control sera.
A
u
t
Proposals to apply the reagents on
o
analysers are available on request.
m
a Each Laboratory has to validate the

t apication in its own responbility.

i
o
n
 1. Buffer and substratecontain sodium acide (0,095%)
Do not swallow avoid contact with skin and mucous
N
membranes.
O 2. During the reaction 5-amino-nitrobenzoate is produ
T ced. This is poisonous when inhaled, swallowed or
when absorbed through the skin. If the reaction
E mixture comes into contact with skin or mucous
membranes, wash with plenty of water.