Professional Documents
Culture Documents
3-Proses Hemopoiesis
3-Proses Hemopoiesis
FORMATION
(HEMATOPOIESIS)
CFUGEMM Lymphoid
stem cell
Erythroid CFUEo
Progenitors CFUMeg CFUGM
CFUE
Thymus
CFU-M CFU-G
B T
lymphotoxins (Table 3)
Table 3. Lymphoid Hematopoietic Growth Factors
Name Source Function
IL - 1 Macrophages, T Stimulates growth of T and B cells,
and B cells, mediates inflammation, inhibits growth of
fibroblasts some cancer cells.
IL - 2 (T T cells Binds to receptors on cytotoxic T cells,
promote their growth, stimulates growth of
cells GF)
helper T cells.
IL - 4 T cells, NK cells, Stimulates & regulates growth and diff. of T
mast cells and B cells, eosinophils and basophils.
IL – 5 T cells Regulates production of eosinophils.
Activates mature eosinophils.
(Eo–GF)
IL - 6 Lymphocytes, Promotes macrophage formation.
fibroblasts Stimulates IgG production by B cells.
IL-7 Stromal Cells Supports long-term survival of B and T
cells in vitro.
IL - 8 Fibroblasts, Enhancement of neutrophil function
vasc. endot.
Common General Features of
Hematopoietic Growth Factors
STEM CELLS
PRONORMOBLAST
BASOPHILIC NORMOBLAST
POLYCHROMATOPHILIC NORMOBLAST
ORTHOCHROMATOPHILIC NORMOBLAST
RETICULOCYTE
~ 2% pronormoblast
~ 18% basophilic normoblast
~ 54% polychromatophilic normoblasts
~ 26% orthochromatophilic normoblasts
Nucleated red cells (normoblasts) appear in the
blood if erythropoiesis is occurring outsides the
marrow (e.medullary e.poiesis) and also with
some marrow diseases.
Erythropoietin (Epo)
Types of leucocytes :
nuclear or mononuclear)
locytes
Maturation stages
MYELOBLAST
PROMYELOCYTE
MYELOCYTE
METAMYELOCYTE
BAND FORM
Functions : ~ Chemotaxis
~ Cytotoxic activities : phagocytosis
and granule toxicity
Life span and circulation :
~ Transit time in blood for mature neutrophils :
1 day or less.
~ Probably survive 1-2 days in tissue.
~ N peripheral blood levels : 4.0 – 10 x 103/ul
2. EOSINOPHILS
Functions :
~ a first line defense against parasites
~ modulate hypersensitivity reactions
~ migrate to inflammatory sites
Life span and circulation :
~ Usually exist in concentr. of 100-400/ul
in PB
~ BM maturation time : 2-6 days ,
circ. half life : 6 - 12 hours
3. Basophils and Mast cells
Functions :
~ involved in immediately hypersensitivity
~ trigger anaphylactic degranulation
~ active arachidonic acid metabolites
(e.g..leukotrienes, prostaglandin)
Life span and circulation :
~ Basophils : about 1-2 days
~ Mast cells : present in blood only in pathologic
conditions. Murine studies : mast cells survive
several weeks to several month in tissue
4. MONOCYTES
Functions : Morphology :
~ Activating stimuli Monoblast
~ Chemotaxis
~ Phagocytosis Promonocytes
Monocytes
Life span :
4.5 – 10 hours (mean 8.5 h)
LYMPHOCYTOPOIESIS
There are three major functional classes of lymphocy-
tes : B lymphocytes (B cell), T lymphocytes (T cell)
and Natural Killer (NK cell)
Differentiation :
~ B - lymphocyte diff. in BM :
Early pre-B cell
pre B-cell
immature B cell
mature B celll
~ T-lymphocyte diff. in the thymus :
Mature T cell
Function :
~ B cell function : humoral immunity (i.e.,anti
body/immunoglobulins production)
Proliferation
CSF-Mega
Thrombopoietin (TP)
THANK YOU