''TRANSFORMATION''

•Transformation in micro-organisms
•Transformation in Animals
•Transformation in plants
•Transformation in prokaryotes
''TRANSFORMATION IN MICRO-
ORGANISMS''
 ''TRANSFORMATION IN MICRO-
ORGANISMS''
Transformation in
E.coli with calcium
chloride
Transformation in
Bacillus by PEG
induced DNA
Transformation in
Yeast and neurospora
by Liposomes
''TRANSFORMATION
IN ANIMALS''
 ''METHODS OF
TRANSFORMATION IN ANIMALS''
DNA coprecipitation with calcium phosphate
DNA complexed with DEAE-dextran
DNA complexed with PEG
Transformation by Micro-injection
‘’DNA comlexed with PEG’’
''MICRO-INJECTION''
 ''DNA COMLEXED WITH
DEAE-DEXTRAN''
DAEA is derivative of carbohydrate polymer
DEXTRAN
DNA trapped with liposoemes
DNA trapped with Erythrocytes ghosts
TRANSFORMATION IN
PLANTS
 ‘’METHODS OF
TRANSFORMATION IN PLANTS’’
''USE OF AGROBACTERIUM
TUMEFECIANS''
''ELECTROPORATION
TECHNIQUE''
''USE OF PARTICLE
GUN''
 ''TRANSFORMATION IN
PROKARYOTES AND LOWER
EUKARYOTES''
''ÇLONING VECTORS''
PLASMIDS
BACTERIOPHAGE
COSMIDS
 ''PLASMIDS''
Plasmids as Extra chromosomal DNA
Role of CO1E1 natural plasmid
use of pBR322 (A derivative of natural
plasmids)
 BACTERIOPHAGE
LAMBDA
Used for screening of
particular DNA sequence
Packaging of recombinant
DNA in labortary
Ease in replication of
recombinant DNA
 ''COSMIDS''
A COSMID is a
plasmid that
contain cos DNA
site and
co1E1DNA
segments.
 ''CLONING VECTORS IN
ANIMALS ''

Vectors derived
from simian virus 40
SV40 as MUTANTS
 ''CLONING VECTORS
IN PLANTS''
DNA plant virion
cauliflower mosaic virus
Agrobacterium
tumefaciens
 ''CROWN GALL
TUMOUR DISEASE''
Caused by PATHOGENIC
BACTERIUM
Agrobacterium tumefaciens
Occur in dicotledunous
plants
 ''THAT’S THE WHOLE
STORY OF
TRANSFORMATION IN
BIOTECHNOLOGY''