Antibiotic Mechanism of Action

Linezolid

Daptomycin
Daptomycin
Linezolid
Daptomycin
inhibitory

bactericidal
MIC = 6.25 mcg/mL
Automated Methods

Many Labs Use

Automated Testing
 E-test®
Kirby-Bauer
Disk Diffusion

Agar dilution
EXAMPLE:
Susceptibility Antibiotic MIC Breakpoint Susceptibility
testing for a single
isolate of Ampicillin >16 8 Resistant
Pseudomonas Gentamicin 2 4 Susceptible
aeruginosa Cephalothin >16 N/A Resistant
Cefepime 8 32 Susceptible
Cefotaxime 16 16/32 Intermediate
-Breakpoint for
intermediate resistance for Ceftazidime 2 32 Susceptible
meropenem is 4 and for
piperacillin/tazobactam Aztreonam 4 16 Susceptible
(pip/tazo) 32
Ciprofloxacin 2 2 Resistant
-Pip/tazo is the better
choice between the two Amp/Sulbactam >16 8 Resistant
-Ciprofloxacin is a poor Meropenem 4 4/8 Intermediate
choice even though the
MIC is lowest of the three
Pip/tazo 8 32-64/128 Susceptible
Decreased
Permeability

Alteration
in Target
Molecule
Antibiotic Degrading Enzymes
Decreased Permeability

Efflux Pumps
Target Alteration
a konjugasi
b  transduksi
c transformasi