Evaluation of antioxidant and antimicrobial activity of Ipomoea mauritiana plant

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and crude fish bile of Pangasius pangasius and Tenualosa ilisha.
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Istiak Alam, AKM Moyeenul Huq*
Department of Pharmacy, School of Science and Engineering
Southeast University, Banani, Dhaka-1213
Abstract Methods and Materials Results (ctd..)
In an attempt to explore the scientific basis for the
Collection of plant Antimicrobial test
pharmacological benefits of the Ipomoea mauritiana and the crude
Ipomoea mauritiana plant were collected from Sokhipur, Mymensing district of Bangladesh.
fish bile extracts of Pangasius pangasius and Tenualosa ilisha the
Preparation of the extract
antioxidant and antimicrobial activities were assessed. Ipomoea
Powdered plant materials are soaked in methanol to get methanolic extract of I. mauritiana.
mauritiana was extracted with methanol and subsequently
Collection of bile samples
analyzed for phytochemical constituents, total phenolic and
The Pangasius pangasius (1–1.5 kg) and Tenualosa ilisha (400–800 gm) were chosen as per availability in the local
flavnoid content. The antioxidant activity was determined by
market for the study. The bile was collected from intact gall bladder of two different fish species separately. The
DPPH free radical scavenging and ferric reducing power activity
collected bile was then preserved at 0ºC for further study.
methods. The antimicrobial activity was evaluated by disk
Collection of microbes
diffusion method and compared with standard kenamycin (30
Tested 4 gram positive and 6 gram negative bacteria's were collected from the Microbiology Lab of Dhaka University
µg/mL). The results revealed that I. mauritiana extract contains
 Antioxidant Study.
tannin, saponin, terpenoids, alkaloid and flavnoids. The total
• The total phenolic content of the extract was determined by the Folin–Ciocalteu method.
pheolic and flavonoid contents of the extract were 59.30 and 27.21
• The total flavonoid content of crude extract was determined by the aluminium chloride colorimetric method.  Ipomoea mauritiana
mg of GAE/gm and QE/gm respectively, while the IC50 of ferric
reducing power activity was 275.08 µg/mL for the methanol
extract as compared to ascorbic acid (IC50, 230.09µg/mL). A  Antimicrobial Study
moderate antibacterial activity was observed for I. mauritiana  Antimicrobial study was done by Disk Diffusion method
methanol extract against all test organisms with the highest zone of
 Pangasius pangasius
inhibition against Bacillius cereus (19.25mm). Similarly, the crude
bile extracts of P. pangasius and T. ilisha showed significant Results
antibacterial activity against all the bacteria of the both strains
where the bile of the earlier species was most active against Anti-oxidant test
Shigella boydii (22.3 mm) and Salmonella paratyphi (22.8mm). T.
ilisha bile on the other side was most active against Vibrio
DPPH free radical scavenging test Ferric Reducing Capacity Test  Tenualosa ilisha
parahemolyticus and Shigella dysenteriae (19.4 and 18.5 mm
respectively). The results indicate that I. mauritiana possesses
considerable antioxidant and antimicrobial property and
P. pangasius, T. ilisha bile have antimicrobial activity.

Background
The World Health Organization (WHO) estimates that as many as
80% of the world’s more than six billion people rely primarily on
animal and plant based medicines.[1] The food-medicine linkages
are relatively new, and much is yet to be discovered from different
cultures, geographic regions and food traditions. In an attempt to
explore the scientific basis for the pharmacological benefits of the
Ipomoea mauritiana and the crude fish bile extracts of Pangasius
pangasius and Tenualosa ilisha the antioxidant and antimicrobial
activities were assessed.
Tasted Samples
a. Ipomoea mauritiana b Pangasius pangasius. c, Tenualosa ilisha
Total Phenolic content test
Here,
C= Total content of phenolic compounds,mg/gm plant extract in GAE
c = The concentration of gallic acid established from the calibration curve (mg/gm)
V=volume of the crude plant extract in gm
Total flavonoid content test
Here,
A= Standard (Kanamycin), B=Blank (DMSO)
C= Tested Sample , D= Sample+DMSO
Discussion
Present study revealed that there was moderate antioxidant activity
and comparatively good antimicrobial activity of I. mauritiana
plant. On the other hand crude bile of P. pangasius has shown
significant antimicrobial activity while T. ilisha bile contains
moderate antimicrobial activities. From the study it is cleared that
fish bile can be a new source of antimicrobial agents.
Conclusions
Further investigation is in necessary to determine the exact
phytoconstituents responsible for antimicrobial effect. It can be
proposed from current study, for further research to perform the
minimum inhibitory concentration (MIC), minimum bactericidal
concentration (MBC), time kill assay and isolate different molecule
from the plant to find out and characterize the secondary metabolites
of the plant and correlate the present finding of the current study.

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