Reproductive technologies

(ప్రత్యు త్ప త్త ి సాంకేత్తతత్)

Dr.I.S.Chakrapani
COMMISSIONERATE OF COLLEGIATE EDUCATION
Government of Andhra Pradesh 1
 The contents..

తృప్త్తమ గర్భ ధార్ణ

(Artificial Insemination)
IVF సాంకేత్తతత్ (IVF Technology)
COMMISSIONERATE OF COLLEGIATE EDUCATION
Government of Andhra Pradesh 2
 Why infertility?
(సాంతాన రాహితాు నికి కార్ణాలు)
1. Anatomical irregularities

(నిరాా ణ లోపాలు)
1. Hormonal irregularities

( హార్మా న్ లోపాలు)
COMMISSIONERATE OF COLLEGIATE EDUCATION 3
Government of Andhra Pradesh
 స్త్ర ి ప్రత్యు త్ప త్తవ్
ి ు వ్స థ
(Female ReproductiveSystem)

4
 Reproductive Cycles -
ప్రత్యు త్ప త్త ి లయలు

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 5
Reproductive Cycles -
ప్రత్యు త్ప త్త ి లయలు

6
 Role of Hormones
హార్మా న్ ల పాప్త్

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 7
 తృప్త్తమ గర్భ ధార్ణ
(Artificial Insemination)
Intra-uterine Insemination -

సిద్ము
ధ చేయబడిన వీర్ు మును స్త్ర ి జీవి
గరాభ శయములో తృప్త్తమముగా
ప్రవేశపెట్టుట
COMMISSIONERATE OF COLLEGIATE EDUCATION
Government of Andhra Pradesh 8
 తృప్త్తమ గర్భ ధార్ణ ఎాందుకు?
( Why Artificial Insemination..?)
Semen Preparation
Controlled ovarian Hyperstimulation
(COH)
Bypassing anatomical barriers
COMMISSIONERATE OF COLLEGIATE EDUCATION
Government of Andhra Pradesh 9
తృప్త్తమ గర్భ ధార్ణ కు సూచనలు
( Indications for Artificial Insemination)
1.Abnormal semen profile
2.Hypospadias, Vaginismus etc.
3.Cervical factors, Tubal defects,
4.Immunological factors
5.Unexplained infertility
COMMISSIONERATE OF COLLEGIATE EDUCATION
10
Government of Andhra Pradesh
 తృప్త్తమ గర్భ ధార్ణ -విధానము
(Artificial Insemination Protocol)
1.రరీక్షాంచుట, నిమాంప్త్ణము
(Physcial Examination & Counselling)
2.హార్మా న్ చికిత్స (Hormone Therapy)
3. వీర్ు మును సిద్ము
ధ చేయుట
(Semen Preparation)
COMMISSIONERATE OF COLLEGIATE EDUCATION
11
Government of Andhra Pradesh
తృప్త్తమ గర్భ ధార్ణ -విధానము
(Artificial Insemination Protocol)
4. వీర్ు మును ప్రవేశపెట్టుట
(Introduction of Sperm)
5. అనాంత్ర్ విప్రాంత్త (Rest)

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 12
Physical Examination & Counselling

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 13
 ప్పాధమిత రరీక్షలు
Physical Examination & Counselling
1. Follicular study

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 14
Physical Examination & Counselling
2. Tubal Status
3. Fibroids, Polyps

Closed fallopian tubes Open tubes Uterine fibroids

COMMISSIONERATE OF COLLEGIATE EDUCATION
Government of Andhra Pradesh 15
Physical Examination & Counselling
4. Hormonal status - PRL, TSH, FSH, LH,
E2, Testosterone, Progesteron

Menstrual Cycle Oestrous Cycle

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 16
 Physical Examination & Counselling
5. Endometriosis, VDRL,HIV
6. Blood sugar, serum insulin, blood group
Semen report for male
7. Both partners should be counselled -
indications, timing & no. of visits, costs
Technique, implications & consent form
COMMISSIONERATE OF COLLEGIATE EDUCATION
Government of Andhra Pradesh 17
 హార్మా న్ చికిత్స
Hormone Treatment - Fertility Drugs
COH aims at increasing the number of oocytes
available for fertilization
1. Oral anti-oestrogens - Clomiphen Citrate
2. Aromatase inhibitors - Letrozole
3. Injectables - FSH
4. hCG - to puncture the follicle & time IUI
COMMISSIONERATE OF COLLEGIATE EDUCATION
18
Government of Andhra Pradesh
Tracking ovulatory cycles ..

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 19
 Preparing the sperm..

1. వీర్ు సేతర్ణ- విశ్ల ేషణ

Semen Collection & Analysis

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 20
Preparing the sperm..

సధార్ణశుప్తతణాలు
COMMISSIONERATE OF COLLEGIATE EDUCATION
Government of Andhra Pradesh 21
Preparing the sperm..

అసధార్ణ శుప్తతణాలు
COMMISSIONERATE OF COLLEGIATE EDUCATION
Government of Andhra Pradesh 22
 Preparing the sperm..
Volume: > 2 mL
Concentration: > 20,000,000 per mL
Motility: > 50%
Normal morphology: > 40% normal
Krueger strict criteria: > 14% normal
Best predictor of fertilizing ability
COMMISSIONERATE OF COLLEGIATE EDUCATION
23
Government of Andhra Pradesh
 Preparing the sperm..

Sperm washing -
one volume mixed with 2 volumes of
culture medium - centrifuged -
supernatant discarded - pellet re-
suspended - centrifuged again

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 24
వీర్ు మునుప్రవేశపెట్టుట
(Insemination)

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 25
 విప్రాంత్త
Rest

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 26
 టెస్ట ు ట్యు బ్ బేబీ
Invitro fertilization (IVF)

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 27
28
 టెస్ట ు ట్యు బ్ బేబీ
Invitro fertilization (IVF)
1. Egg collection
2. Sperm collection
3. Fertilization
4. Implantation
5. Surrogacy

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 29
 టెస్ట ు ట్యు బ్ బేబీ
Invitro fertilization (IVF)

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 30
Ham’s F10 Medium
Earl’s Solution
Modified Whitten’s Medium
Whittingham’s T6 Medium
10000-50000 motile sperm are added
to @1 ml of medium
COMMISSIONERATE OF COLLEGIATE EDUCATION 31
Government of Andhra Pradesh
At the time of M Il oocyte with one polar 2 PN Embryo
retrieval body

4 Cell Embryo 8 cell Embryo Full grown blastocyst

COMMISSIONERATE OF COLLEGIATE EDUCATION
Government of Andhra Pradesh 32
 టెస్ట ు ట్యు బ్ బేబీ
Invitro fertilization (IVF)

COMMISSIONERATE OF COLLEGIATE EDUCATION

Government of Andhra Pradesh 33