CLSI Guidelines

What, How, When and Why?

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Clinical and Laboratory Standards Institute (CLSI)

International, interdisciplinary, non-profit, standards

developing and educational organisation
Develops standards and guidelines by means of consensus
Covers all aspects of the clinical laboratory
 Laboratory design
 Specimen collection
 Clinical Chemistry
 Haematology
 Immunology
 Microbiology
 Quality Control
 Etc.

www.clsi.org
Microbiology Standards Institutes

Clinical and Laboratory Standards Institute (CLSI)

European Committee on Antimicrobial Susceptibility
Testing (EUCAST)
British Society for Antimicrobial Chemotherapy (BSAC)
Japanese Society of Chemotherapy (JSC)
Antimicrobial Susceptibility Testing (AST) Standards

M100-S22 Performance Standards for Antimicrobial

Susceptibility Testing; Twenty-Second Informational
Supplement
M02-A11 Performance Standards for Antimicrobial Disk
Susceptibility Test; Approved Standard – Eleventh Edition
M07-A9 Methods for Dilution Antimicrobial Susceptibility
Tests for Bacteria That Grow Aerobically; Approved Standard
– Ninth Edition

Anaerobic Susceptibilities
Mycobacteria, Nocardia and Actinomyces susceptibilities
Breakpoint Revisions
Screening and Confirmation Tests
Antibiotic Selection For Routine Susceptibility
Testing

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Recommended Antibiotics to Test
Recommended Antibiotic Groups
Group A – Test and Report Routinely
Group B – Report Selectively
Group C – Test and report Selectively
Group U – Urinary Tract Infections
CSF
Drug Deductions
Warnings
Testing Conditions and Minimal Quality Control
General Comments
Breakpoints
Staphylococci Screening Tests
Quality Control Tables

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Quality Control – Zone Diametres (mm)
Quality Control – MIC (ug/ml)
Quality Control Strains
Quality Control Frequency of Testing
Quality Control Troubleshooting
Intrinsic Resistance

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Intrinsic Resistance

Intrinsic resistance is defined as inherent or innate (not

acquired) antimicrobial resistance, which is reflected in
wild-type antimicrobial patterns of all or almost all
representatives of a species.
Intrinsic resistance is so common that susceptibility
testing is unnecessary. For example, Citrobacter
species are intrinsically resistant to ampicillin.
Intrinsic Resistance - Enterobacteriaceae
Intrinsic Resistance

Provides a way to evaluate the accuracy of testing

methods
Aids in the recognition of common phenotypes;
Assist with verification of cumulative antimicrobial
susceptibility test data.
An “R” occurring with an organism-antimicrobial
combination means that strains should test
resistant.
A small percentage (1%–3%) may appear
susceptible due to method variation, mutation, or
low levels of resistance expression.
In effective Antibiotics In Vivo
Antibiotic Classification Tables

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Antibiotic Classification
Class and Sub-class
Routes of Administration
Duplicate Abbreviations
Related Standards
Introduction
Our R&D Expertise

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Introduction

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Introduction
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Introduction
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CONCLUSION
YOUR PARTNER FOR CREATING
THE FUTURE OF MICROBIOLOGY

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