Monochromatic Staining

Mr. Pradip Bawane

Assistant Professor
SVKM’s Institute of Pharmacy, Dhule
 Monochromatic staining
• Principle:
• Staining with single dye solution contain salts.
• Auxochrome and chromophore.
• Auxochrome responsible for the dissociation of
the dye and a chromophore responsible for the
colour of the dye.
• In basic dye chromophore is positively charge
and the cell have negative charge.
• Ex. Methylene blue, crystal violet etc.
 Monochromatic Staining

Crystal
violet Wash with Distilled
water

Dry the Slide

Observe Under
Microscope
(100X)
THANK YOU