Visible Light Microscope

1. Bright field Microscopy

2. Dark field Microscopy
3. DIC (Difference Interference Contrast)
4. Phase Contrast Microscopy
5. Polarisasi Microscopy
 BRIGHT FIELD MICROSCOPY
• Most commonly used microscopy imaging technique is bright field
microscopy, where light is either passed through or reflected off a specimen
• Biologists and histologists have used counter staining for over one hundred
years; and this helps to differentiate the various tissues and organelles that
can be found in a variety of subjects that would otherwise be rendered
invisible

Drawbacks:
The cells are usually killed and therefore cannot be studied while moving
around their natural habitat

When to use bright field microscopy

• Viewing stained or naturally pigmented specimens such as stained prepared
slides of tissue sections
• Used when there is enough contrast in the subject matter or artificial staining
techniques are employed .
DISADVANTAGE OF BRIGHT FIELD
 BRIGHT FIELD

Main uses:
• Viewing stained specimens
• Pathological exams
• Blood tests
• Water inspections
• Liquid crystal board inspections
 Bright field Requirements

Condenser

Objectives
 Blue Filter
 Illumination being emitted by Halogen Lamp
tends to be on the Yellow Hue. Colour of image
are being influence by it. Thus, it tend to be
yellowish.

 A Blue filter is required to compensate or to

correct the colour of this error.
Thank you!