TOM ANTHONY A.

TONGUIA, RMT
 *Junqueira’s Basic Histology
 Di Fiore’s Atlas of Histology
 4 term examinations (Prelim, Midterm,
Prefinal, Final)
 The examination comprises 50 items written,
and the other 50 is practical exam (moving
exam)
 Students will be informed of their scores.
 Final practical examination is a
comprehensive exam
 Absences and tardiness – 30 mins. grace
 Complete uniform (except washday),
labgowns
 Electronic gadgets
 Cell phones, camera, laptop, tablets, recorders
NOT ALLOWED
 Cheating in any form is NEVER
TOLERATED
 SENDMAE HUNA, RMT- LECTURE
 TOM ANTHONY A. TONGUIA, RMT-
LABORATORY
HISTOLOGY-lab
 Histology
 Greek word “histo”- means tissue or web
 Branch of morphological science that deals with
the study of
 Many cell types and extracellular components
 Tissues arranged into organs
 Cells – tissues - organ
 Cytology

 Study of cells
 Cells spontaneously shed off
 Cells physically removed by swabs or brushings
 Microscope

 Comes from the Greek word “mikros” and

“skopein”.
 A compound light microscope is used to view
minute details of cells and primarily tissues.
 Compound
 Light
 Bright-field microscopy
 - widely used; composed of mechanical and
optical parts
 -examined by means of light that passes
through the specimen
 Uses amount of ultraviolet light to observe the
image.
 Acridine Orange- example of fluorescent stain
 - uses a lens system that produces visible
images from transparent objects
 For tissue cultures and observing living cells.
 DIM- produces an image w/ a more apparent
3D aspect than phase contrast
 Avoids stray light and achieves greater resolution
by using:
 (1) small point of high intensity light provided by
laser and
 (2) a plate w/ a pinhole aperture in front of image
detector
 Allows the recognition of stained and
unstained structures made of highly organized
molecules.
 Birefringence- ability to rotate the direction of
vibration of polarized light. A feature of
crystalline substances
 Transmission electron microscopy- permits
resolution around 3mm allowing up to 400,000
times magnification
 Scanning electron microscopy- permits pseudo
3D images of surfaces of cells