Oxidase test

Tetramethyl paraphenylene
diamine dihydrochloride
Simmon’s citrate agar
 Indole test
Para dimetyl
aminobenzaldehyde
Motility test
Agar is 0.2%
Urease test
Kligler’s Iron Agar
This test is used to test whether an organism can
produce and maintain acid end products in a medium. In
effect this is a quantitative test for acid production from
fermentation of glucose.

Methyl red test

The purpose of this test is to determine whether an organism can
produce acetylmethylcarbinol (acetoin) from fermentation of glucose.
The culture is incubated in phosphate glucose medium. Half of this
medium is then used for the methyl red test and the other half for a
Voges-Proskauer test. Alpha-naphtol (5%) and potassium hydroxide
(40%) are added to the the medium. If acetoin is present the medium
.will turn pink-reddish in colour
Voges Proskauer test
Ortho-nitrophenyl-b-D-galactopyranoside )
Lysine iron agar