MENGENAL PATOGENITAS SAR COV-2

Sri Darmawati
Juni 2020
coronavirus disease 2019….COVID-19

Wuhan City Seq.

novel Corona
Hubey Provinsi Virus genom
SARS- identik
Cina
(2019- CoV-2
(Desember
nCov) SARS-
2019)
CoV

Februari 2020 ada 25 negara

≥ 70.000 kasus infeksi Pneumonia associated respiratory syndrome

Real time Merusak

Reverse CT paru-paru
Transcription radiogra
PCR..RT-PCR phy
M E

S
N

M • Membran
glycoprotein

S • Spike Surface protein

• Envelope
E
SARS-CoV-2
• The virion is roughly 90–120 nm in diameter.
• Virus RNA
• Envelope positif
• Mengandung lipid bilayer
• Genom dilindungi oleh nukleocapsid yang tersusun helix
• The genome size is about 30 kb in length and encodes 15
open reading frames (Orfs)
• Major Orfs encode four structural proteins
1. spike (S) protein (180/ 90 kDa),
2. envelope (E) protein (8 kDa),
3. membrane (M) protein (23 kDa),
4. nucleocapsid (N) protein (50–60 kDa)
SARS-CoV-2
• approximately 16 nonstructural proteins (nsp1–16),
• and five to eight accessory proteins
•.
ACE2 diekspresikan oleh:
1. human lung alveolar epithelial cells (type I and type II
pneumocytes),
2. enterocytes of the small intestine,
3. brush borders of the proximal tubular cells of the kidney,
4. endothelial cells of arteries and veins,
5. arterial smooth muscle cells in several organs
(Hamming et al. 2004).
Proses masuknya SARS-CoV-2 pada host

1. Dimediasi oleh surface spike protein, Mirip HIV dan

influenza
Binding dan fusi pada permukaan sel host
hemaglutinin
2. The functional receptor for the spike protein of SARS-CoV is
a metallopeptidase, angiotensin-converting enzyme 2 (ACE2)
(Li et al.2003)
3. It comprises an N-terminal S1 subunit responsible for
virus–receptor binding and a C terminal S2 subunit
responsible for virus– cell membrane fusion
4. Kemudian genom RNA dilepaskan, gabung dengan ribosom
sel host. The positive-sense viral RNA genome can serve as 
messenger RNA and can be translated into protein in the 
host cell
5. Translasi…hasilnya protein polimerase milik virus
• A negative-sense single-stranded RNA
virus (or (-)ssRNA virus) is a virus that uses 
negative sense, single-stranded RNA as its genetic
material.

• The negative viral RNA is complementary to the mRNA

 and must be converted to a positive RNA by 
RNA polymerase before translation.

• Negative sense ssRNA viruses need RNA polymerase to

form a positive sense RNA. The positive-sense RNA acts
as a viral mRNA, which is translated into proteins for the
production of new virion materials. With the newly formed
virions, more negative sense RNA molecules are
produced.
VIRUS RNA
• 1. SARS-COV-2
• 2. Dengue virus
• 3. Hepatitis C virus
• 4. HIV-type 1 virus
• 5. Polio virus
Lanjutan
6. RNA mengalami replikasi
Sintesis N protein
7. Sintesis S protein
Sintesis pada ribosom RE
Sintesis E protein
Sintesis M protein
8. Penyempurnaan pada badan golgi
9. Asembliy dan budding
10. Mature virion
11. Virus release
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Trends in Immunology, May 2020, Vol. 41, No. 5

Perspectives on the development of neutralizing antibodies
against SARS-CoV-2