SCREENING OF MEDICINAL PLANT CRUDE

EXTRACT/ FRACTIONS FOR DIFFERENT INVIVO

AND INVITRO PHARMACOLOGICAL ACTIVITIES

SUBMITTED BY
BUSHRA ANSARI

RESEARCH SUPERVISOR
DR.MEHBOOBALAM

Department of Pharmaceutical Sciences,

Abbottabad University of Science and Technology
Session 2015-2017

 
INTRODUCTION
Healing with medicinal plants is as old as mankind
itself.
Man’s search for drugs to cure diseases can be
traced back to stone-age; the evidence of which can be
found in written documents, preserved monuments,
and even original plant medicines.
Awareness of medicinal plants usage is a result of
mankind’s battle against illnesses due to which he
learned to pursue drugs in barks, seeds, fruit bodies,
and other parts of the plants.
 Cont…
Traditional medicines have always played a key role
in world health and continue to be used to treat a wide
range of conditions and complaints.
Wide varieties of traditional medicinal plants,
growing on productive land of Pakistan, are
unknowingly exploited and have not been
scientifically investigated for their biologically active
principles.
According to a survey of Pakistan, about 6000
species of flowering plants have been existing.
 Cont…
After going through the literature survey, I will select
a plant from genus Kalancho of Crassulaceae family
 Crassulaceae is morphologically distinct and
taxonomically abstruse family (orpines or stonecrop)
comprises of 1400–1500 species, classified in 33
genera.
Genus Kalanchoe includes 139 species native to
Southern and Eastern Africa, and, Tropical Africa,
Pakistan and India.
 Cont…
Different species of Kalanchoe are indicated in
folklore and are used traditionally in treatment of
fever, abscesses, wounds, coughs, skin infections,
hypertension, rheumatism and inflammatory diseases.
AIMS AND OBJECTIVES
The specific objectives of this study would be:
Extraction of plant material to obtain crude drug.
To evaluate the crude drugs by organoleptic methods
using qualitative and quantitative techniques.
Fractionation of crude extract.
 Cont…
To perform the different phyto-pharmacological
activities of crude extract and subsequent fractions.
The ultimate objective of this study is to investigate
the medicinal plant which is not explored for their
potential as medicines for different conditions.
 METHODOLOGY
The research will be conducted in different phases.
Phase 1: Complete literature survey on these species
will be carried out.
Phase 2: Collection, drying, pulverizing, extraction and
fractionation of the plant material.
Phase 3: Different pharmacological invitro activities like
antibacterial, and different invivo activities like analgesic,
antidairrhoeal, antiemetic and antidepressant, activities
will be performed by using the approved pharmacological
techniques/ methods.
 MATERIAL AND METHODS
Collection and Extraction of Plant
Collection of plant
Shade drying
Pulverization
Extraction
Fractionation
 Cont…
Phytochemical Analysis
Different qualitative and quantitative tests will be
performed to identify group of organic compounds such as
alkaloids, saponins, terpenes, flavones, lignans etc
Pharmacological Activities
The crude extract of plant material will be screened for the
pharmacological activities.
PHARMACOLOGICAL ACTIVITIES
Antibacterial Activity:
Antibacterial activity will be performed by using agar-well
diffusion method.
Analgesic:
Hot plate method and Acetic Acid-induced Writhing
Method will be used to evaluate analgesic activity.
Anti-diarrheal:
Anti diarrheal activity will be assessed by charcoal
propulsion method.
 Cont…
Antiemetic:
Copper sulphate induced emesis method will be used
for antiemetic activity.
Antidepressant:
Force swimming test will be used for antidepressant
activity.
 Cont…
Animals:
BALB/c healthy mice (male) and pigeons will be used for
different activities. Animals will be fed on the general
laboratory food stuff and fresh water ad libitum. The
animals will be maintained under defined laboratory
environment at temperature of 25 oC ± 2 oC.
SOCIOECONOMIC BENEFITS OF THE STUDY

From this research study, I expect positive results from the

plant crude extract that will help provide scientific evidence
to the claim of traditional healers and provide another safe
and effective agent to the drug discovery.
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