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  • RS Kanker Dharmais RQAP

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    Wahyu Nirwanto
    7 views7 pages
    The document outlines the protocol for an RQAP Pdl-1 test conducted at RS Kanker Dharmais hospital in Jogjakarta, Indonesia on October 18, 2019. The 13-step protocol includes deparaffinization, rehydration, antigen retrieval using Tris EDTA at pH 9, blocking steps, overnight primary antibody incubation, secondary antibody detection, DAB staining, hematoxylin counterstaining, dehydration and clearing of slides. The detection kit used was an UltraTek HRP Anti-polyvalent system from Scytek Laboratories. Potential troubleshooting steps and interpretation guidelines are also provided.

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    The document outlines the protocol for an RQAP Pdl-1 test conducted at RS Kanker Dharmais hospital in Jogjakarta, Indonesia on October 18, 2019. The 13-step protocol includes deparaffinization, rehydration, antigen retrieval using Tris EDTA at pH 9, blocking steps, overnight primary antibody incubation, secondary antibody detection, DAB staining, hematoxylin counterstaining, dehydration and clearing of slides. The detection kit used was an UltraTek HRP Anti-polyvalent system from Scytek Laboratories. Potential troubleshooting steps and interpretation guidelines are also provided.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    7 views7 pages

    RS Kanker Dharmais RQAP

    Uploaded by

    Wahyu Nirwanto
    The document outlines the protocol for an RQAP Pdl-1 test conducted at RS Kanker Dharmais hospital in Jogjakarta, Indonesia on October 18, 2019. The 13-step protocol includes deparaffinization, rehydration, antigen retrieval using Tris EDTA at pH 9, blocking steps, overnight primary antibody incubation, secondary antibody detection, DAB staining, hematoxylin counterstaining, dehydration and clearing of slides. The detection kit used was an UltraTek HRP Anti-polyvalent system from Scytek Laboratories. Potential troubleshooting steps and interpretation guidelines are also provided.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 7

    Laporan RQAP Pdl-1

    RS Kanker Dharmais

    Jogjakarta, 18 Oktober 2019


    PROTOKOL
    1. Deparafinisasi
    2. Rehidrasi
    3. Pre treatment dengan Tris EDTA pH 9 microwave low 30 menit
    4. Peroxidase Block
    5. Super Block
    6. Antibody Primer PD-L1 1 : 25 2x overnight
    7. Ultratek Anti-Polyvalent
    8. Ultratek HRP
    9. DAB Solution 1 : 20
    10. Hematoxylin
    11. Bluing Reagen
    12. Dehidrasi
    13. Clearing
    Rumah Sakit Kanker Dharmais 2019
    Detection Kit

    Ultratek HRP Anti-polyvalent (DAB) Staining Complete System

    Scytek Laboratories

    Rumah Sakit Kanker Dharmais 2019


    KENDALA

    Rumah Sakit Kanker Dharmais 2019


    TROUBLESHOOTING

    Rumah Sakit Kanker Dharmais 2019


    INTERPRETASI

    Rumah Sakit Kanker Dharmais 2019


    TERIMA KASIH

    Rumah Sakit Kanker Dharmais 2019

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