Construction and Screening of

DNA Libraries
A genomic DNA library is a set of DNA
clones collectively containing the entire
genome of an organism.

A cDNA library contains the coding regions

of the expressed genes of an organism. It is
made of complementary DNA (cDNA)
synthesized from RNA by reverse
transcriptase.
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 Screening DNA Libraries for Genes of
Interest
• Genetic Selection—searching for a DNA
sequence that restores the wild-type
phenotype to a mutant organism.
• Molecular hybridization is based on the
hybridization of labeled similar DNA
sequences (probe).
– Radioactive
– Chemiluminacence

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 Colony
Hybridization

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Eukaryotic Messenger RNA (mRNA)
It is possible to isolate only mRNA from total RNA
(remove rRNA, tRNA, and other types of RNA)

Bead or column
Synthesis of Double-Stranded cDNAs
from mRNA

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