MY PROJECT

Isolation and identification of new microalgae strains with antibacterial,

activity on food-borne pathogens. Engineering approach to optimize
synthesis of desired metabolites
 ABSTRACT
This paper presents a strategy for isolation, identification and
evaluation of microalgae strains synthesizing biologically active
compounds (BAC) with antimicrobial activity against food-borne and
other pathogens. Water samples containing microalgae were initially
submitted to the enrichment process, followed by standard procedure
for obtaining pure culture. Purified DNA from the microalgae isolates
was used for sequencing the amplified 18S rDNA genomic region,
which resulted in the identification of Poterioochromonas
malhamensis, Chlorella sp., Micractinium sp., Tetradesmus sp. and
Desmodesmus sp.
All isolated strains were cultivated in 1-L flat-plate PBRs, with the
Poterioochromonas malhamensis being the only one to show
antibacterial activity in agar diffusion assay. Further, PPEQ-01strain
was cultured under different types and scales of PBR, intensity and
spectrum of the incident light, in order to study and prove the
antibacterial potential of the strain. Ethanolic and methanolic
supernatants, or hydrophilic and methanolic extracts produced from
biomass cultivated in different conditions led to formation of bacterial
growth inhibition halos around the impregnated disks used in the agar
diffusion test.
The hydrophilic extract, obtained from
biomass cultured under green
illumination, exhibited stronger activity
against all tested pathogenic bacteria as
compared to the other extracts. The
results obtained with the new isolated
Poterioochromonas malhamensis strain
proved efficiency of the innovative
complex approach applied to BAC
aiming the identification of microalgae
that present antimicrobial activity.
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