HISTOLOGICAL FEATURES OF CANDIDIASIS

BY:MAMDOUH DAGSH ALSHRIFI

ID:321103259
 Objective

 CANDIDIASIS
 types of candidiasis
 Histological Features
 References
 CANDIDIASIS
Candida albicans is an opportunistic fungal pathogen that
is responsible for candidiasis in human hosts.
 C. albicans grow in several different morphological
forms, ranging from unicellular budding yeast to true
hyphae with parallel-side wall .
Candida albicans is a unicellular, oval-shaped diploid
fungus (a form of yeast )

Typically, C. albicans live as harmless commensals in the

gastrointestinal and genitourinary tract and are found
in over 70% of the population. Overgrowth of these
organisms, however, will lead to disease
Candidiasis is a fungal infection that can affect
areas such as the:
Skin
Genitals
Throat
Mouth
Blood
 THERE ARE SEVERAL TYPES OF
:CANDIDIASIS
If it is in the mouth or throat, it is called oral
candidiasis, oropharyngeal candidiasis, or thrush.
If it affects the genital area, it is called a yeast
infection. In women, it may be called a
vulvovaginal yeast infection.
If yeast infects the skin on a baby's bottom area,
it causes a diaper rash.
If the infection enters bloodstream, it is called
invasive candidiasis or candidemia
 HISTOLOGICAL FEATURES

• ACUTE:
 NEUTROPHILS IN THE STRATUM CORNEUM IS THE CHARACTERISTIC FEATURE.  
 DIFFUSE INFLAMMATORY INFILTRATES AND INTRAEPITHELIAL MICROABSCESSES. 
 FUNGAL ELEMENTS ARE SPARSE AND DEMONSTRATED WITH THE PAS STAIN.
CHRONIC:
 MARKED HYPERKERATOSIS AND PSEUDOEPITHELIOMATOUS HYPERPLASIA.
 COMPACT ORTHOKERATOSIS AND SCALE CRUST FORMATION. 
 SPORES AND HYPHAE ARE EASILY IDENTIFIED EVEN WITHOUT PERIODIC ACID-SCHIFF (PAS) STAINS. 
 GRANULOMATOUS DERMATITIS :  ILL-DEFINED GRANULOMAS COMPOSED OF LYMPHOCYTES,
PLASMA CELLS, EPITHELIOID CELLS AND OCCASIONALLY GIANT CELLS.
 HISTOLOGICAL FEATURES OF
CANDIDIASIS (FIGURE 1)

Histopathological Findings Of Papilloma (A: Without Candidiasis, B:

With Candidiasis) And
Verrucous Hyperplasia (C: Without Candidiasis, D: With Candidiasis).
“C” Means
Candidiasis In The Figures.
Papillary And Verrucous Outward Proliferation With Parakeratotic
Stratified
Squamous Epithelium Was Observed In Papilloma (Fig.1-a) And
Verrucous
Hyperplasia (Fig.1-c), Respectively.
Pseudohyphae Or Hyphae Penetrated Vertically Into The Keratinized
Layer Or Upper
Layer Of The Prickle Layer Of Papilloma With Candidiasis (Fig.1-b).
Neutrophilic Infiltration Was Observed In The Connective Tissue And
Was Invaded In
The Epithelial Tissue (Fig.1-d). PAS Reaction Revealed Clearly Hyphal
Invasion (Inset
In Fig.1-b And Fig.1-d).
HISTOLOGICAL FEATURES OF CANDIDIASIS
(FIGURE 2)
Longitudinal Section Of Candidal Hyphe (HL) Was Existed Within The Oral

Epithelium (E). Cross Section Of Candidal Hyphe (HC) Internalized Into The

Epithelial Cell Inside A Cytoplasmic Vacuole (V). The Cell Covered With Plasma

Membrane (PM) Is Surrounded By Thick Cell Wall (CW). (Fig.2-1:x5,000).

Candial Hypha Containing Mitochondrias (M) And A Nuclear (N) Had Invaded

Along An Intercellular Bridge Was Observed. Desmosomal Cell Junction (D) Of The

Epithelial Cells (E) And Dense Tonofilament (TF) Were Arranged Irregularly In The

Host Cytoplasm. Destruction Of Desmosomal Cell Junction (Arrow) And Deficit Of

Tonofilament (Arrowhead) Were Also Observed At The Tip Of The Hypha

(Fig.2-2:x10,000). “C” Means Candidiasis In The Figures.

 HISTOLOGICAL FEATURES OF CANDIDIASIS (FIGURE 3)

In The Without Candidiasis Group, A Strong Positive Reaction Was

Seen Across The
Whole Of The Prickle Layer, Which Presented A Clearly Scalar
Appearance (Figs.3-a,c).
In The Candidiasis Group, Weaker Expression Was Seen In The Prickle
Layer (Figs.3-b
(1),d(1)). Strong Positive E-cadherin Reaction Was Seen Along The
Insertion Sites Of

The Hyphae (Figs.1-b(2),d(2), Arrowhead). “C” Means Candidiasis In

The Figures.
 HISTOLOGICAL FEATURES OF
CANDIDIASIS (FIGURE 4)
CK13 Was Negative In The Basal Layer Of Papilloma And
Verrucous Hyperplasia
Without Candidiasis (Figs.4-a1,c1). In Papilloma And
Verrucous Hyperplasia With
Candidiasis, Weakly Positive In The Basal Layer And
Strongly Positive In The Whole
Prickle Layer. (Figs.4-b1,d1).
Egfr Was Almost Negative In Without Candidiasis Groups
(Figs.4-a2,b2,c2,d2).
Weakly Positive Egfr Reactions Were Observed In The
Upper Layer Of The Prickle
Layer In Papilloma And Verrucous Hyperplasia With
Candidiasis (Figs.4-b2,d2).
Higher Values For Ki-67 Positivity Were Found In Both The
Basal Layer And The
Prickle Layer Of The Candidiasis Group (Figs.4-b3,d3)
Than The Without Candidiasis
Group (Figs.4-a3,c3). “C” Means Candidiasis In The
Figures.
 HISTOLOGICAL FEATURES OF CANDIDIASIS
(FIGURE 5)
NE And CD68 Were Almost Completely Negative The Without
Candidiasis Group
(Figs.5-a1,c1,a2,c2). In Papilloma And Verrucous Hyperplasia
With Candidiasis,
Strongly Positive Reactions Were Seen On The Prickle Layer And
Connective Tissue
(Figs.5-b1,d1,b2,d2).
Cox-2 Was Negative In The Without Candidiasis Group (Figs.5-
a3,c3). In The
Candidiasis Group, COX-2 Was Positive In All Layers In
Papilloma (Fig.5-b3), And Mix
Of Strongly And Weakly Positive Reaction Was Seen In Verrucous
Hyperplasia
(Fig.5-d3).
In The Investigation Of New Blood Vessels Using Cd105 (Figs.5-
a4,b4,c4,d4),
Numerous Enlarged Blood Vessels Were Found In The Connective Tissue
Immediately
Below The Basal Membrane In Papilloma (Fig.5-b4) And Verrucous
Hyperplasia With
Candidiasis (Fig.5-d4). “C” Means Candidiasis In The Figures.
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