Muhammad Nawaz Shareef University of Agriculture, Multan

Institute of Plants Breeding and Biotechnology

Internship Presentation
• Presented by: M Zeeshan Zafar
• Supervisor Dr. M. Mahmood Ahmed

3/6/21 12:16 PM Institute of Plant Breeding & Biotechnology 1

Contents
Introduction
Objectives
Field visits
Identification of wheat varieties
Data collection
To check Individual trait performance
Statistical analysis
ANOVA
Correlation
Results and conclusion

03/06/2021 Institute of Plants Breedinng and Biotechnology

Introduction
• wheat one of the first domesticated food crops
• Basic staple food of the major civilizations for last 8000 years
• Source of calories
• Protein
• Certain vitamins
• Minerals

03/06/2021 Institute of Plants Breedinng and Biotechnology

Objectives
To assess the genetic diversity in the genotype under study

To assess the nature and magnitude of inter-character

correlation

To identify best performing varieties related to individual traits

By Using statistics 8 .1 to perform statistical analysis

03/06/2021 Institute of Plants Breedinng and Biotechnology

Field visits
Identification of wheat varieties
 Galaxy-2013
 Faisalabad-2008
 Ujala-2013
 Gandum-1
 Jauhar-2006
 Fakhar-e-bakhar
 Millat
 Punjab-2011

03/06/2021 Institute of Plants Breedinng and Biotechnology

Data Collection

• Plant Height
• Spike length
• Grain/spike
• 1000grain weight
• Grain length
• No. of tillers
• NO. of spikelet's/spike

03/06/2021 Institute of Plants Breedinng and Biotechnology

Collected Data

1000
varieties P.H S.L G/S G.W G.L T/P s/s
Galaxy 2013 102 11 52 40 0.8 5 21
Faisal abad 2008 82 9 48 38 0.8 7 20
Jauhar 2016 109 13 48 39 0.7 5 20
Ujala 2013 103 11 51 38 0.8 5 21
Gandum 1 103 12.5 54 38 0.6 6 22
Sahar 2006 82 8.5 50 37 0.6 4 19
Fakhr e Bakhar 102 11 55 37 0.7 6 23
Millat 2011 99 9 47 39 0.7 4 19
Punjab 2011 100 8 48 37 0.8 5 20

03/06/2021 Institute of Plants Breedinng and Biotechnology

Collected Data
120

100

80

60 P.H
S.L
G/S
1000 G.W
40 G.L
T/P
F/S
20

0
13 08 16 13 1 06 ar 11 11
0 20 0 0 um 20 kh 0 20
y2 d r2
al a2 nd ar eB
a t2 ab
lax ba uh
a a h li la nj
Ga la a Uj G Sa r u
Ja akh M P
is F
Fa

03/06/2021 Institute of Plants Breedinng and Biotechnology

 Individual trait performance
Plant height Spikelet’s/spike
30
120
100 25
80 20
60 15
40 plant.height 10 Spikelets/spike
20
5
0
3 8 6 3 1 6 r t b 0
01 200 200 201 um- 200 kha illa nja t
y-
2
d- r- a- d r- ba m pu 0 13 008 006 013 m-1 006 har illa njab
lax aba uha ujal gan aha re- 2 2 2 2 u
y- d- ar- la- nd ar- -ba
2 k m pu
ga sal ja s kh la ax laba uh uja ga sah hre
iF a fa g sa ja k
fa
Fia

spike length 1000grain weight

14
60
12 50
10 40
8 30
6 20
spike length
4 10
1000grain
2 0 weight
0 t
0 13 008 006 013 m-1 006 har illa njab
t k
0 13 008 006 013 m-1 006 har illa njab 2 2 2 2 u
y- d- ar- la- nd ar- -ba
2 m pu
y-
2
d-
2 -2 -2 d u r-2 -ba
k m pu la ax laba uh uja ga sah hre
ar jala an aha g sa ja k
la ax laba uh g r e fa
g sa ja u s kh Fia
fa
Fia

03/06/2021 Institute of Plants Breedinng and Biotechnology

Cont.
Grain/spike
56
54
52
50
48
46
grain/spike
44
42
t
0 13 008 006 013 m-1 006 har illa njab
2 2 2 2 u 2 k m pu
y- d- ar- la- nd ar- -ba
la ax laba uh uja ga sah hre
g sa ja fa
k
Fia

Grain length
0.9
0.8
0.7
0.6
0.5
0.4
0.3 grain length
0.2
0.1
0
3 ar t
1 08 06 013 m-1 006 illa nj
ab
20
- -20 r-20 -2 u -2 akh m u
y d a d ar -b p
lax laba uha ujal ga
n
ah khre
ga sa ja s
fa
Fia

03/06/2021 Institute of Plants Breedinng and Biotechnology

Statistical analysis

 Software use
• Statistics 8.1
Test
• Descriptive statistics

• Analysis of variance(ANOVA)
• Correlations

03/06/2021 Institute of Plants Breedinng and Biotechnology

Descriptive statistics
Variable N Mean SD Minimum Maximum
G.L 9 0.7222 0.0833 0.6000 0.8000
G.S 9 50.333 2.8723 47.000 55.000
G.W 9 38.222 1.0929 37.000 40.000
N.T 9 5.2222 0.9718 4.0000 7.0000
P.H 9 97.778 9.2841 82.000 108.00
S.L 9 10.333 1.7854 8.0000 13.000
S.S 9 20.111 1.1667 19.000 22.000
geno 9 5.0000 2.7386 1.0000 9.0000

03/06/2021 Institute of Plants Breedinng and Biotechnology

Analysis of variance
AOV Table for Plant height
Source DF SS MS F P
rep 1 2.72 2.722
geno 8 1012.78 126.597 23.68 0.0001
Error 8 42.78 5.347
Total 17 1058.28

Grand Mean 97.389 CV 2.37

AOV Table for Spike length
Source DF SS MS F P
rep 1 0.8889 0.88889
geno 8 56.5278 7.06597 7.19 0.005
Error 8 7.8611 0.98264
Total 17 65.2778

Grand Mean 10.111 CV 9.80

03/06/2021 Institute of Plants Breedinng and Biotechnology
Cont.
AOV Table for Grain per spike

Source DF SS MS F P
rep 1 0.222 0.2222
geno 8 133.111 16.6389 8.44 0.0034
Error 8 15.778 1.9722
Total 17 149.111

Grand Mean 50.222 CV 2.80

AOV Table for 1000 grain weight

Source DF SS MS F P
rep 1 8.290E-31 8.290E-31
geno 8 16.1111 2.01389 5.37 0.0142
Error 8 3.00000 0.37500
Total 17 19.1111

Grand Mean 38.222 CV 1.60

03/06/2021 Institute of Plants Breedinng and Biotechnology

Cont.
AOV Table for Spikelet's per spike
Source DF SS MS F P
rep 1 1.411E-30 1.411E-30
geno 8 11.7778 1.47222 2.94 0.0739
Error 8 4.00000 0.50000
Total 17 15.7778

Grand Mean 20.111 CV 3.52

AOV Table for No. of tillers

Source DF SS MS F P
rep 1 5.5556 5.55556
geno 8 10.0000 1.25000 4.09 0.0313
Error 8 2.4444 0.30556
Total 17 18.0000

Grand Mean 4.6667 CV 11.85

03/06/2021 Institute of Plants Breedinng and Biotechnology

Correlations
P.H S.L G/S 1000 G.W G.L T/P F/S
P.H 1
S.L 0.690* 1
G/S 0.256* 0.487* 1
1000 G.W 0.375* 0.409* -0.178* 1
-
G.L 0.094* -0.224* 0.295** 0.252* 1
T/P -0.108* 0.240* 0.325* -0.149* 0.240* 1
F/S 0.454* 0.568* 0.892** -0.138* -0.012* 0.568* 1

03/06/2021 Institute of Plants Breedinng and Biotechnology

Conclusions
• From the results of analysis of variance of data

• From the results of correlation data

• From the results of individual traits performance

03/06/2021 Institute of Plants Breedinng and Biotechnology

 Acknowledgement
 
Dr. Mahmood Ahmed
Assistant professor
IPBB, MNSUA,Multan
 THANK YOU

03/06/2021 Institute of Plants Breedinng and Biotechnology