Procalcitonin: Uses in the Clinical

Laboratory for the Diagnosis of Sepsis

Leader: Angela Jane Asube

Members: Marjory Gedaro
Hannah Vae Tayco
Louie Mae Cuizon
Kirkjan Royce Fajardo
PROCALCITONIN: USES IN THE CLINICAL
LABORATORY FOR THE DIAGNOSIS OF SEPSIS

SIGNIFICANC
E METHOD

INTRODUCTION TECHNIQUE DISCUSSION

 INTRODUCTION

Sepsis is the systemic response to infection

by microbial organisms. A differential
diagnosis of infection caused by either
bacteria or other microbial organisms is
essential for effective treatment and
prognostic assessment. In this article, we
review the current knowledge of PCT and its
use in the clinical laboratory setting.
SIGNIFICANCE

Identifying whether the cause of

inflammation in patients is of bacterial
origin has been an important area of
development in the clinical laboratory. An
accurate and timely diagnosis of sepsis
allows prompt and appropriate treatment.
TECHNIQUE
Time Resolved Amplified Cryptate
Emission (TRACE) Technology

Non-radiative energy transfer from the donor

to the acceptor, which is part of a chemically
modified, light-collecting algal protein, is
the basis of the TRACE technology.

BENEFITS
-Precise results
-Less waste
-Faster results
-No wash and separation step
-Homogenous immunoassay
TECHNIQUE
The donor (europium
cryptate) recognize
the epitopes of
immature CT region.

Procalcitonin binds and it The acceptor

triggered by a nitrogen (fluorophore) absorb
laser at 337 nm. light at a particular
wavelength.

Upon excitation, it emits signal

The donor is the one to receive
at 665 nm that result to
the light and upon excitation, it
amplified signal and prolonged
emits (produce) flourescent
that last for a few micro-
signal at 620nm.
seconds.
DISCUSSION

There have been a number of clinical laboratory tests to diagnose

sepsis however it's either they take too long to diagnose or they
lack specificity. In this article the focus was on PCT, a useful
biomarker for patients suffering from sepsis.

There have been a lot of evidence suggesting that monitoring

PCT can reduce the morbidity and mortality of patients with
sepsis. Although its use in diagnosing inflammation caused by
bacterial origins look promising, there are still reports wherein its
levels does not coincide with bacterial infections. Further studies
in determining the role of PCT and the pathogenesis of sepsis is
needed.
THANKS!
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