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Microorganisms of The Skin and Mucous Membranes
Microorganisms of The Skin and Mucous Membranes
2. Pathogenic microorganisms
Role of resident flora of the skin and mucous membranes:
- Suppression of the normal flora creates a partial local void that tend to be
filled by microorganisms from the environment or from other part of the
body.
- Such organisms behaves as opportunists and may be become pathogen
Role of the resident flora
• Staphylococcus epidermidis
• Staphylococcus aureus ( in small numbers )
• Alpha-hemolytic and nonhemolytic Streptococcus
• Micrococcus species
• Peptostreptococcus species
• Neisseriae species ( nonpathogenic )
• Propionibacterium species
• Diphtheroids
• Candida species ( small numbers )
• Acinetobacter species ( small numbers )
Genus Staphylococcus
. Cells spherical, 0,5 – 1,5 um in diameter, occurring singly, in pair and irregular
cluster
• Gram positive, nonmotile, nonsporing
• Facultative anaerobic, chemoorganotrophic,with both respiratory and fermentative
metabolism
• Colonies are usually opaque and may be white or cream and sometimes yellow to
orange
• Usually catalase positive, cytochromes present but usually oxidase negative
• Nitrate often reduce to nitrite, susceptible to lysis by lysostaphin but not by
lysozyme
• Usually grow with 10 % NaCl, the optimum temperature is 300-370C
• Mainly associated with the skin and mucous membranes of warm-blooded
vertebrates, but are often isolated from food products, dust and water
• Some species produce extracellular toxin
Tabel 1. Laboratory test for differentiation of
Staphylococcal sp.
MSA :
-Growth + + +
-Fermentation + - -
Coagulase + - -
DNase + - -
Hemolysis beta - -
Novobiocin S S R
test
Pigmentation Golden white white
yellow
Genus Streptococcus
• Cocci are 0,6 1,0 um in diameter, occurring singly but more often in
pairs with adjacent sides flattened; one species (N. elongata) is an
exception and consists of short rods 0,5 um wide, often arranged as
diplobacilli or in short chains
• Division of the coccal species is in two planes at right angle to each
other, sometimes resulting in tetrads
• Capsules and fimbriae (pili) may be present; endospore are not
present
• Cells stain Gram negative, but there is tendency to resist
decolorization
• Swimming motility does not occur, and flagella are absent
• Aerobic; some species produce a greenish yellow carotenoid pigment
Genus Neisseria
• Pleomorphic rods, 0,5-0,8x1-5 um, are often club shaped with one
end rounded and the other tapered; some cells may be coccoid, bifid
or branched, but they are not filamentous; cells occur singly, in pairs
or short chains, in V or Y configurations, or in clumps with “chinese
character” arrangement
• Gram positive, non motile, nonsporing
• Facultative anaerobes but have variable aerotolerance; most grow
somewhat in air but better anaerobically, giving on blood agar
colonies that are usually convex, semi opaque, glistening, and often
pigmented in shades of cream to reddish
• Chemoorganotrophic with complex nutritional requirement, have a
metabolism fermentative, producing from glucose and some other
carbohydrates large amounts of propionic acid , acetic acid and often
small amounts of gas
Genus Propionibacterium
, Conjunctiva
• Mouth and upper respiratory tract
• Intestinal tract
• Urethra
• Vagina
Normal flora of the conjunctiva
• Diphtheroids
• Staphylococcus epidermidis
• Streptococcus (nonhemolyticus)
• Neisseriae
• Moraxella sp
• The mucous membranes of the mouth and pharynx are often sterile at
birth but may be contaminated by passage through birth canal
• Within 4 – 12 hours after birth, viridans streptococci become
established as the most prominent members of the resident flora and
remain so for life. They probably originate in the respiratory tracts of
the mother and attendants.
• Early in life, aerobic and anaerobic staphylococci, gram negative
diplococci ( Neisseriae, Moraxella catarrhalis ), diphtheroids, and
occasional lactobacilli are added
Normal flora of the mouth and upper respiratory tract
• Nonhemolytic streptococcus
• Alpha hemolytic streptococcus
• Neisseriae
• Staphylococci
• Diphtheroid
• Haeomophili
• Pneumococci
• Mycoplasma
• Prevotella
Normal flora of the mouth and upper respiratory tract
- Corynebacteria
- Staphylococcus epidermidis
- Staphylococcus aureus
- Streptococci
Normal flora of the intestinal tract
• Synthesis of vitamin K
• Conversion of bile pigments and bile acids
• Absorption of nutrients and breakdown products
• Antagonism to microbial pathogens
• The intestinal flora produces ammonia and other breakdown products
that are absorbed and can contribute to hepatic coma
• Among aerobic coliform bacteria, only few serotypes persist in the
colon for prolonged periods, and most serotypes of Escherichia coli
are present only over period of a few days
Normal flora of the intestinal tract
• The anterior urethra of both sexes contains small numbers of the same
types of organisms found on the skin and perineum
• This organism regularly appear in normal voided urine in numbers of
102 – 104/mL
Normal flora of the vagina
Staphylococcus aureus
Impetigo :
• An easily spread exudative infection of the epidermis occurring
primarily in children; may results in nephritis as complications
• Should be treated with penicillin and scratching should be prevented
Cellulitis and erysipelas :
• Initiated by infection through a small break in the skin
• The term cellulitis applies if the lesion is defined
• Erysipelas applies if the lesion spreads, primarily through the
lymphatic Fasciitis :
• A rapidly spreading infection of the fascia; tends to occur in diabetic
patients; needs surgical debridements of necrotic tissue and therapy
with antibiotics
Clostridium perfringens
Cellulitis :
• Occur in patient with burns, wound, or neutropenia; may be highly
necrotic; indicated by blue-green pus and grape-like sweet odor
Septicemia :
• Results from hematogenous spread of infection from local lesion or
gastrointestinal tract and causes gram negative shock
• May result in a distinctive lesion, ecthyma gangrenosum, when
dermal veins and tissue are invaded. These lesions become necrotic
Diagnosis