Professional Documents
Culture Documents
Objectives/Learning Outcomes
Describe , discuss , the principle and application of Aseptic Technique
Understand and be able to explai the use of the Laminar Flow Hood
Appreciate the sources of contamination and how to minimise product cont
Lecture Flow
Definitions
Background
Laminar Air Flow Hoods
Aseptic Technique Applications
• Enteral
– Of or relating to the intestines
(e.g. by mouth, orally, per tube)
• Parenteral
– Introduced other than by way of the intestines
(e.g. intravenous, intramuscular, subcutaneous)
• Aseptic Technique
– The technique for manipulations of compounded sterile products
and parenteral preparations that prevents contamination
Filtered Air
Room Air
Prefilter
• Physical Structure
– Hard shell or soft shell
• Hard shell (plastic, Plexiglas, stainless steel)
• Soft shell (soft plastic film)
• Internal Environment
– Less airflow required to achieve ISO 5 (Class 100) conditions
– Entering and exiting air is to be HEPA filtered
– Isolators for cytotoxic preparations should capture vapor
– Positive pressure maintained for non-chemotherapy products
– Negative pressure is maintained for chemotherapy products
• Monitoring Systems
• Gauges to monitor positive pressure environment
• Surface sampling for contamination
NEVER TOUCH
Tip or Plunger
1.5ml measured
• Hub
– Where the needle attaches
to the syringe tip and
allows the fluid in the
syringe barrel
• Bevel
– The tip of needle is slanted
to a point and the slanted
part of the needle is the
bevel. The bevel allows for
smooth insertions through
stoppers and ports with
minimal coring
• Coring
– The development of a core
or hole in the rubber of a
vial
– To prevent coring insert
needle as shown
– Insert the bevel tip first,
then pressing downward
and toward the bevel so
the bevel tip and heel enter
at the same point
Vials Ampules
• To prevent contamination
– Swab rubber closure with 70% alcohol using firm strokes in the
SAME direction
• To prevent core formation
– Insert needle to penetrate the rubber closure at same point with
both tip and heel of bevel
• To prevent vacuum formation
– Inject an equal amount of air for the volume of fluid to be
removed
• Reconstituting drug powder
– Remove an equal amount of air for the volume of diluent added
• To break ampule
– Clean ampule neck with alcohol swab
– Leave swab in place
– Grasp ampule neck with thumb and index finger
– Use quick, firm, snapping motion away from body towards side
wall of hood
– DO NOT BREAK TOWARD HEPA FILTER
3 4
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