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Public Health Emergency Operations Center
PPP 3.1: IMPORTANCE HAND HYGIENE
FOR PREVENTING NCOV
Hand washing
Antiseptic Hand-rub
WHO. 2005. Guidelines on Hand Hygiene in Health Care (Advanced Draft): A Summary. WHO: Geneva
Formulation 1:
• To produce final concentrations of ethanol 80% v/v, glycerol
1.45% v/v, hydrogen peroxide (H2O2) 0.125% v/v:
• Pour into a 1,000-mL graduated flask:
• Ethanol 96% v/v, 833.0 mL
• H2O2 3%, 41.7 mL
• Glycerol 98%, 14.5 mL
• Top up the flask to 1,000 mL with distilled water or water
that has been boiled and cooled; shake the flask gently to
mix the contents.
Formulation 2:
• To produce final concentrations of isopropyl alcohol 75% v/v,
glycerol 1.45 v/v, hydrogen peroxide 0.125% v/v:
• Pour into a 1,000-mL graduated flask:
• Isopropyl alcohol (with a purity of 99.8%), 751.5 mL
• H2O2 3%, 41.7 mL
• Glycerol 98%, 14.5 mL
• Top up the flask to 1,000 mL with distilled water or water that
has been boiled and cooled; shake the flask gently to mix the
contents.
• v/v=volume percent, meaning 80 parts absolute alcohol in
volume and 20 parts water measured as volume, not as weight
Add 100ml 70% Alcohol with 2ml 99% Glycerin and mix
thoroughly