Development of an Enzyme-Linked Immunosorbent Assay

for Rapid Detection of Dengue Virus (DENV) NS1 and

Differentiation of DENV Serotypes during Early Infection

PRESENTED BY,
Fahima Akter (ID: 2018-2-76-092)
Samia Afroje Rafia(ID: 2018-2-77-043)
Bushra Altaf Chowdhury( ID: 2015-3-77-002)
Nazim Uddin(ID: 2018-2-77-008)
Sumona Islam( ID: 2018-2-77-011)
Introduction:
-Caused by Aedes aegypti and Aedes albopictus.

-Four serotypes.

-(ELISA) for detection of Dengue virus NS1 by pairing a serotype-cross-reactive monoclonal antibody (MAb) with one
of four serotype-specific MAbs

-Rapid detection of the dengue virus & distinguish specific serotypes.

-146 serum samples was obtained.

-Sensitivity rate of test - 84.85%.

-Sensitivity rates for serotyping : 88.2% (15/17) for DENV serotype 1 (DENV1), 94.7% (18/19) for DENV2, 75%
(12/16) for DENV3, and 66.6% (6/9) for DENV4.
Materials & Methods:
Results:
Results:
Discussion:
-Native form enhance the ability to recognize NS1 proteins in patient serum

-.Sensitive, with the lowest detectable concentration

-Able to discriminate the four dengue virus serotypes without cross reactivity

- Able to identify the specific serotype with a high degree of specificity

-Capable of detecting DENV NS1 antigens rapidly in the early stage

-Beneficial in regions where medical resources are limited

-Facilitate mass serotyping during emerging epidemics

-Used to identify distributions of various DENVs along with the contributing factors

- Improve epidemiological surveillance, outbreak monitoring, clinical management, and knowledge of the serotypes

Future Prospective

-Analysis of patient sera infected with dengue, Japanese encephalitis,, Zika virus, or West Nile virus.

-Development of an immunochromatographic rapid test