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  • Diagnosis Penyakit TNM (Bakteri)

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    Ratna arifiani S
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    Diagnosis of plant diseases involves identifying the disease through its symptoms and characteristic signs, as well as other related factors. Correct diagnosis is needed to recommend proper control measures and for plant disease surveys. The diagnosis process involves isolating the causal organism from a diseased plant and testing Koch's postulates to confirm that it causes the disease. Laboratory tests are then used to identify the organism, such as culturing it from plant tissues or using molecular methods like PCR.

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    Materi bakterio

    Original Title

    1. DIAGNOSIS PENYAKIT TNM (BAKTERI)

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    Diagnosis of plant diseases involves identifying the disease through its symptoms and characteristic signs, as well as other related factors. Correct diagnosis is needed to recommend proper control measures and for plant disease surveys. The diagnosis process involves isolating the causal organism from a diseased plant and testing Koch's postulates to confirm that it causes the disease. Laboratory tests are then used to identify the organism, such as culturing it from plant tissues or using molecular methods like PCR.

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    4 views10 pages

    Diagnosis Penyakit TNM (Bakteri)

    Uploaded by

    Ratna arifiani S
    Diagnosis of plant diseases involves identifying the disease through its symptoms and characteristic signs, as well as other related factors. Correct diagnosis is needed to recommend proper control measures and for plant disease surveys. The diagnosis process involves isolating the causal organism from a diseased plant and testing Koch's postulates to confirm that it causes the disease. Laboratory tests are then used to identify the organism, such as culturing it from plant tissues or using molecular methods like PCR.

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    of 10

    DIAGNOSIS PENYAKIT

    • Diagnosis ialah suatu proses untuk


    mengidentifikasi suatu penyakit tanaman melalui
    gejalanya dan tanda penyakit yang khas
    termasuk faktor-faktor lain yang berhubungan
    dengan proses penyakit tersebut.
    • Diagnosis penyakit yang benar diperlukan untuk
    merekomendasikan cara pengendalian yang
    tepat dan dalam suatu survei tanaman penyakit
    tanaman.
    • Organisme tertentu harus
    didapat dalam inang yang
    ada.
    • Organisme ini harus
    diisolasikan dan dibuat
    biakan murni.
    • Jika diinokulasikan kepada
    inang sehat yang sama ,
    organisme tersebut harus
    menghasilkan penyakit
    yang sama.
    • Organisme tersebut harus
    didapatkan lagi dari inang
    tadi dalam biakan murni.
    Postulat Koch
    Laboratory tests

    Fungal isolation
    Moist Incubation  Surface sterilize.
     Goal – to induce sporulation  Use margin of diseased area.
     Important for obligate fungal  Nutritive agar media, can be
    pathogens selective
     Avoid overly moist conditions.  Diagnostician can often ID to
     Can surface sterilize genus from appearance of
    culture
    Laboratory tests Isolate identification
    Bacterial identification
     Biochemical tests
     Carbohydrate utilization
    (BIOLOG)
     Fatty acid methyl ester analysis
    (FAME)
     Molecular methods
     Polymerase chain reaction (PCR)
     Serological tests, e.g. ELISA

    Ralstonia solanacearum

    Bacterial isolations
     Grind tissue in sterile water.
     Streak suspension on bacterial
    medium.
     Can use selective media
     Transfer single, isolated colony
    Identification of other pathogens
    ELISA tests
    Multi-well test for
    multiple samples

    Individual ELISA
    kit
    Link diagnosis bakteri patogen tanaman
    https://www.youtube.com/watch?v=nCDMgOFmWKM
    https://www.youtube.com/watch?v=eLHFN-Zvgps
    https://www.youtube.com/watch?v=-iSfrg2Gn0k
    https://www.youtube.com/watch?v=BwNa4nfTZ-A
     

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