VACCUM LIQUID

CHROMATOGRAPHY
VACUUM LIQUID CHROMATOGRAPHY

ANI SM
MECH
 ADSORPTION

 DESORPTION

 ELUTION

 DIFFERENT OF PRESSURE
Size of adsorbent particle

CC (Kolom klasik) KCV :

63 – 250 μm 40 - 63 μm

TLC
40 - 63 μm
Elution Method

 Based on

Using various of eluent

by increasing polarity
VLC eluent

N-hexane Ethyl acetat methanol Total volume

9 1 25 ml
8 2 25 ml
7 3 25 ml
dst
1 9 25 ml
10 0 25 ml
9 1 25 ml

dst dst
0 10 25 ml
Penyiapan adsorben

 Adsorben dimasukkan ke dalam kolom

 Tinggi adsorben setelah vakum 5 -6 cm

Contoh soa;
 Jika diketahui berat ekstrak yang akan dikromatografi
kolom 12 g, berat jenis silika gel H-60 adalah 0,7211
g/ml, diameter kolom 8 cm. Berapakah tinggi kolom
setelah di vakum jika kolom dikemas dengan silika gel
dengan perbandingan ekstrak : silika gel = 1 : 15
Preparing sample

Sample is prepared in powder form

Sample + adsorbent
Collecting fraction

Collecting fraction

Each Fraction
Fraction monitoring

TLC PC

FRACTIONS THAT CONTAIN THE SAME

COMPONENT ARE COMBINED
MEDIUM PRESSURE
LIQUID
CHROMATOGRAPHY
MEDIUM PRESSURE LIQUID
CHROMATOGRAPHY

:
MECHANISM
 ADSORPTION
 DESORPTION
 ELUTION
 GRAVITATION
 PRESSURE 50 – 150 psi
1 atm = 14,7 psi
Size of adsorbent particle

CCC
63 – 250 μm

VLC
40 - 63 μm

MPLC
40 - 63 μm
ELUTION METHOD

 ISOCRATIC

 GRADIENT
Collecting fraction

 Automatically by machine

a s e d on
B
 Volume

 Time
Fraction monitoring

TLC PC

FRACTIONS THAT CONTAIN THE SAME

COMPONENT ARE COMBINED