Professional Documents
Culture Documents
and
Flow Cytometry
To detect and
measure
multiple
properties of
particles or
cells for
identification
and
quantitation.
● The light from the laser hits the single particle and
scatters in different directions.
Forward
Scatter
LASER
Side Scatter
Types of Light Scatter
Forward Scatter
Side Scatter
Image from Peripheral Blood Smear Evaluation
Fluorochromes
Photomultiplier C
Cell Tube P
U
● If the cell has the antigen that the antibody (Ab) Antigen
(target
specifically binds (target molecule), the antibody molecule)
and fluorochrome attach to the cell.
Cell
Immunophenotyping with Flow Cytometry
● NO TARGET MOLECULE NO
FLUORESCENCE Cell
References